CIT 抗体 (AA 470-1505)
This 兔 多克隆 antibody specifically detects CIT in ELISA, WB 和 FACS. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN7875167
发货至:
中国
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Quick Overview for CIT 抗体 (AA 470-1505) (ABIN7875167)
抗原
See all CIT 抗体
CIT
(Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT))
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This CIT antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB), Flow Cytometry (FACS)
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抗原表位
- AA 470-1505
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原理
- CIT Antibody / Citron Rho-interacting kinase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human CIT recombinant protein (Position: Q470-H1505) was used as the immunogen for the CIT antibody.
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亚型
- IgG
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anti-Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT) (AA 1928-2027) antibody
CIT 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT) antibodyCIT 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the CIT antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the CIT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- CIT (Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT))
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别名
- CIT
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背景
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CIT antibody detects Citron Rho-interacting kinase, a serine/threonine kinase that regulates cytokinesis and neuronal development. CIT functions downstream of Rho family GTPases to coordinate actomyosin contractility and midbody formation during cell division. The CIT antibody is used in cell cycle, cytoskeletal, and neurodevelopmental research to study contractile ring organization and cytokinetic abscission.
CIT is encoded by the CIT gene located on human chromosome 12q23.1. The protein is approximately 230 kilodaltons and contains an N-terminal kinase domain, a coiled-coil region, a Rho-binding domain, and a C-terminal citron homology region with multiple protein-protein interaction motifs. CIT localizes to the midbody during cytokinesis and to dendritic spines in postmitotic neurons.
The CIT antibody detects the 230 kilodalton protein in western blot analysis and reveals distinct midbody and dendritic staining patterns. CIT acts as a scaffold and kinase that phosphorylates substrates including myosin light chain and actin-regulatory proteins, coordinating contraction during cell division. Depletion of CIT disrupts cleavage furrow ingression and leads to multinucleation due to failed cytokinesis.
In the nervous system, Citron kinase contributes to dendrite formation, axon stability, and synaptic maturation. Mutations in CIT cause microcephaly and neuronal migration disorders due to abnormal mitotic spindle dynamics and impaired cell cycle progression in neural progenitors. CIT also interacts with RhoA and other GTPases, integrating cytoskeletal signaling with cortical actin organization.
CIT is a critical effector of Rho-mediated contractility and has been implicated in cancer cell proliferation and migration. NSJ Bioreagents provides a validated CIT antibody optimized for its applications, enabling detailed studies of cytokinesis, cytoskeletal remodeling, and neurodevelopmental regulation. -
UniProt
- O14578
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途径
- SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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