PNKP 抗体 (AA 43-487)
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Quick Overview for PNKP 抗体 (AA 43-487) (ABIN7874971)
抗原
See all PNKP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 43-487
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原理
- PNKP Antibody / Polynucleotide kinase phosphatase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PNKP recombinant protein (Position: D43-E487) was used as the immunogen for the PNKP antibody.
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亚型
- IgG
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anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 60-320) antibody
PNKP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 422-521) antibodyPNKP 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-521) antibodyPNKP 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB965 unconjugated Recombinant Antibody
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) antibodyPNKP 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB805 unconjugated Recombinant Antibody
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (N-Term) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 1-243) antibodyPNKP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (AA 359-408) antibodyPNKP 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Polynucleotide Kinase 3'-Phosphatase (PNKP) (N-Term) antibodyPNKP 适用: 人, 小鼠, 犬, Cow, 豚鼠, 马, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PNKP antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PNKP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))
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别名
- PNKP
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背景
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PNKP antibody detects Polynucleotide kinase phosphatase, a DNA repair enzyme that restores damaged DNA termini during base excision and single-strand break repair. PNKP possesses both 5'-kinase and 3'-phosphatase activities, preparing DNA ends for ligation by DNA ligase. The PNKP antibody is widely used in DNA repair, neurobiology, and cancer research to study genome maintenance, oxidative damage response, and repair pathway coordination.
PNKP is encoded by the PNKP gene on human chromosome 19q13.33. The protein is approximately 521 amino acids long and contains an N-terminal forkhead-associated (FHA) domain that mediates interactions with XRCC1 and XRCC4, scaffolding proteins in the single-strand and double-strand break repair complexes. The C-terminal catalytic region includes both phosphatase and kinase domains that process DNA termini with abnormal 3'-phosphate or 5'-hydroxyl ends.
The PNKP antibody detects a 57 kilodalton band by western blot and shows nuclear foci following DNA damage. PNKP plays a dual role in the repair of oxidative and radiation-induced DNA breaks by preparing strand ends for gap filling and ligation. Mutations in PNKP cause neurological disorders such as microcephaly with early-onset seizures and developmental delay, resulting from defective DNA repair in postmitotic neurons.
PNKP activity is tightly regulated by phosphorylation and interaction with repair complexes. In cancer, overexpression of PNKP enhances DNA repair capacity, contributing to therapy resistance. Pharmacologic inhibition of PNKP sensitizes tumor cells to radiation and chemotherapeutic agents, underscoring its potential as a therapeutic target.
Because PNKP maintains genomic stability and neuronal survival, it serves as a key model for studying the interface between DNA repair and neurodegeneration. NSJ Bioreagents provides a validated PNKP antibody optimized for western blot, immunofluorescence, and DNA repair assays, supporting detailed analysis of genome maintenance and repair coordination. -
UniProt
- Q96T60
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途径
- DNA Damage Repair, Nucleotide Phosphorylation
抗原
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