WHSC2 抗体 (AA 38-482)
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Quick Overview for WHSC2 抗体 (AA 38-482) (ABIN7874510)
抗原
See all WHSC2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 38-482
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原理
- NELFA Antibody / Negative elongation factor A
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NELFA recombinant protein (Position: I38-L482) was used as the immunogen for the NELFA antibody.
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亚型
- IgG
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anti-Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2) (AA 280-511) antibody
WHSC2 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 6B11H8 unconjugated
anti-Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2) (AA 280-511) antibodyWHSC2 适用: 人, 大鼠 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 6B11H8 unconjugated
anti-Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2) (AA 280-511) antibodyWHSC2 适用: 人, 大鼠 WB, ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2) (AA 1-528) antibodyWHSC2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2) (AA 499-549) antibodyWHSC2 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2) (AA 429-527) antibodyWHSC2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2) (AA 280-511) antibodyWHSC2 适用: 人 WB, ELISA, IF, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 6B11H8 unconjugated
anti-Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2) (AA 499-549) antibodyWHSC2 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NELFA antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NELFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))
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别名
- NELFA
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背景
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NELFA antibody detects Negative elongation factor A, a transcriptional regulator that controls RNA polymerase II pausing and transcription elongation. Encoded by the NELFA gene on chromosome 9q34.11, this protein is a core component of the negative elongation factor (NELF) complex, which also includes NELFB, NELFC/D, and NELFE subunits. Together, these proteins transiently inhibit RNA polymerase II progression downstream of transcription start sites, enabling proper promoter-proximal pausing, RNA capping, and regulated gene expression.
NELFA interacts with DSIF (DRB sensitivity-inducing factor) and phosphorylated RNA polymerase II to modulate transcriptional timing and elongation efficiency. Release from pausing is mediated by P-TEFb-dependent phosphorylation events that allow productive elongation. Through these mechanisms, NELFA coordinates transcriptional responses to developmental, environmental, and signaling cues.
The NELFA antibody is widely used in transcription, chromatin, and epigenetic regulation research to study RNA polymerase II pausing, elongation control, and transcriptional activation. Western blot analysis identifies a 66 kilodalton band corresponding to NELFA, while immunofluorescence reveals nuclear punctate staining associated with active transcription foci. This antibody supports studies investigating transcriptional regulation and pause-release mechanisms.
Altered NELFA activity affects global gene expression programs, contributing to developmental disorders, cancer, and viral replication control. NELFA has also been linked to stress responses, where it modulates transcription restart following DNA damage or heat shock. The NELFA antibody provides a critical reagent for dissecting the molecular mechanisms of transcription elongation and RNA polymerase II regulation. NSJ Bioreagents offers this antibody validated for its applications, ensuring dependable performance in transcriptional research. -
UniProt
- Q9H3P2
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途径
- Chromatin Binding
抗原
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