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MCM4 抗体 (AA 361-677)

The 兔 多克隆 anti-MCM4 antibody is suitable to detect MCM4 in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IF, IHC (p) 和 FACS.
产品编号 ABIN7874331
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MCM4 抗体 (AA 361-677) (ABIN7874331)

抗原

See all MCM4 抗体
MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

适用

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人, 小鼠, 大鼠

宿主

  • 100
  • 6
  • 3

克隆类型

  • 90
  • 19
多克隆

标记

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  • 6
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  • 3
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This MCM4 antibody is un-conjugated

应用范围

  • 87
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  • 3
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  • 1
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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
  • 抗原表位

    • 15
    • 8
    • 6
    • 6
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    • 5
    • 4
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    • 3
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 361-677

    原理

    MCM4 Antibody

    纯化方法

    Antigen affinity

    免疫原

    Recombinant human partial protein (amino acids 361-677) was used as the immunogen for this MCM4 antibody.

    亚型

    IgG
  • 应用备注

    The stated application concentrations are suggested starting amounts. Titration of the MCM4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

    限制

    仅限研究用
  • 状态

    Lyophilized

    缓冲液

    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

    储存条件

    4 °C,-20 °C

    储存方法

    After reconstitution, the MCM4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
  • 抗原

    MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

    别名

    MCM4

    背景

    Minichromosome maintenance, S. cerevisiae, homolog of, 4, also called CDC21, is a protein that in humans is encoded by the MCM4 gene. MCM4 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The 864-amino acid protein has an observed molecular mass of 97 kD by SDS-PAGE, which is similar to its calculated molecular mass of 96.6 kD. Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells. In the absence of DDK, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial. The gene encodes a subunit of the MCM2-7 complex (also known as MCM2-MCM7), the replication licensing factor and presumptive replicative helicase.

    UniProt

    P33991

    途径

    DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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