POLM 抗体 (AA 36-481)
This 兔 多克隆 antibody specifically detects POLM in WB, ELISA 和 IP. It exhibits reactivity toward 人.
产品编号 ABIN7874321
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for POLM 抗体 (AA 36-481) (ABIN7874321)
抗原
See all POLM 抗体
POLM
(Polymerase (DNA Directed), mu (POLM))
适用
人
宿主
兔
克隆类型
多克隆
标记
This POLM antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA, Immunoprecipitation (IP)
-
-
抗原表位
- AA 36-481
-
原理
- POLM Antibody / DNA polymerase mu
-
纯化方法
- Immunogen affinity purified
-
免疫原
- E.coli-derived human POLM recombinant protein (Position: E36-H481) was used as the immunogen for the POLM antibody.
-
亚型
- IgG
-
-
anti-Polymerase (DNA Directed), mu (POLM) (C-Term) antibody
POLM 适用: 人, 小鼠, 马, Pig, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal dilution of the POLM antibody should be determined by the researcher.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- After reconstitution, the POLM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
-
-
- POLM (Polymerase (DNA Directed), mu (POLM))
-
别名
- POLM
-
背景
-
POLM antibody detects DNA polymerase mu, a specialized DNA polymerase involved in non-homologous end joining (NHEJ) and DNA repair. The UniProt recommended name is DNA polymerase mu (POLM). This enzyme belongs to the X family of DNA polymerases, functioning at sites of double-strand breaks to fill in short gaps and process damaged DNA ends during repair.
Functionally, POLM antibody identifies a 494-amino-acid nuclear enzyme that exhibits template-dependent and template-independent polymerase activities. POLM incorporates nucleotides opposite damaged or missing bases, ensuring ligation competency of DNA ends. Unlike high-fidelity polymerases, POLM lacks strong proofreading activity, allowing it to tolerate mismatched or gapped templates-a property critical for repair flexibility in NHEJ.
The POLM gene is located on chromosome 7p13 and is expressed in immune and proliferative tissues such as thymus and bone marrow. It plays a key role in V(D)J recombination and class switch recombination in lymphocytes by repairing programmed double-strand breaks. Its activity complements DNA polymerase lambda (POLL) in filling in short DNA overhangs during repair.
Pathologically, dysregulation of POLM contributes to genomic instability and may influence cancer development, immune function, and cellular aging. Overexpression can increase mutation rates, whereas deficiency impairs NHEJ efficiency. POLM's balance between flexibility and error rate is vital for maintaining genome integrity while allowing adaptability in immune diversification.
POLM antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect DNA polymerase mu in nuclear extracts. NSJ Bioreagents provides POLM antibody reagents optimized for DNA repair, recombination, and genomic maintenance research.
Structurally, DNA polymerase mu contains an N-terminal BRCT domain mediating protein-protein interactions, followed by a catalytic polymerase domain with palm, fingers, and thumb subdomains typical of X-family enzymes. This antibody supports studies on POLM's mechanistic role in end joining, mutation repair, and adaptive immunity. -
UniProt
- Q9NP87
-
途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
-
You are here:
