STK39 抗体 (AA 346-376)
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Quick Overview for STK39 抗体 (AA 346-376) (ABIN7874119)
抗原
See all STK39 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 346-376
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原理
- SPAK Antibody / STK39
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纯化方法
- SAS precipitation
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免疫原
- A portion of amino acids 346-376 from the human protein were used as the immunogen for the SPAK antibody.
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亚型
- Ig Fraction
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应用备注
- The stated application concentrations are suggested starting points. Titration of the SPAK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the SPAK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- STK39 (serine threonine Kinase 39 (STK39))
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别名
- SPAK
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背景
- SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.
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UniProt
- Q9UEW8
抗原
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