MYLK3 抗体 (AA 34-812)
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北京 101111
Quick Overview for MYLK3 抗体 (AA 34-812) (ABIN7874092)
抗原
See all MYLK3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 34-812
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原理
- MYLK3 Antibody / Myosin light chain kinase 3
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human MYLK3 recombinant protein (Position: E34-K812) was used as the immunogen for the MYLK3 antibody.
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亚型
- IgG
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anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 40-69), (N-Term) antibody
MYLK3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3066 unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 40-69) antibodyMYLK3 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 555-786) antibodyMYLK3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) antibodyMYLK3 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 1-240) antibodyMYLK3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) antibodyMYLK3 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 488-750) antibodyMYLK3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (Internal Region) antibodyMYLK3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 1-240) antibodyMYLK3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase 3 (MYLK3) (AA 535-562) antibodyMYLK3 适用: 人 WB 宿主: 兔 Polyclonal RB31393 unconjugated
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应用备注
- Optimal dilution of the MYLK3 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the MYLK3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))
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别名
- MYLK3
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背景
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MYLK3 antibody targets Myosin light chain kinase 3, a cardiac-specific serine/threonine kinase that phosphorylates the regulatory light chain of myosin II to modulate cardiac muscle contraction. The MYLK3 gene encodes cardiac myosin light chain kinase (cMLCK), which plays a pivotal role in sarcomere assembly, contractile force generation, and maintenance of cardiac output. cMLCK phosphorylates myosin regulatory light chain (MLC2v), enhancing the interaction between actin and myosin filaments to promote efficient contraction of cardiomyocytes.
MYLK3 expression is restricted mainly to cardiac muscle tissue, distinguishing it from other members of the myosin light chain kinase family such as MYLK and MYLK2, which function in smooth and skeletal muscle, respectively. Located on chromosome 16q23.1, the MYLK3 gene is activated during cardiac differentiation and is transcriptionally regulated by cardiac transcription factors including NKX2-5 and GATA4. Its kinase domain shares structural homology with other Ca2+/calmodulin-dependent kinases but exhibits distinct substrate specificity suited to cardiac myosin regulation.
Functional studies demonstrate that MYLK3 enhances cardiac contractility by maintaining phosphorylation of ventricular myosin light chain 2 (MLC2v). Loss-of-function mutations or reduced expression of MYLK3 have been linked to dilated cardiomyopathy and impaired sarcomere organization. Overexpression of MYLK3 increases calcium sensitivity and promotes sarcomere maturation during cardiomyocyte development, emphasizing its importance in cardiac physiology. In experimental models, altered MYLK3 signaling can influence cardiac hypertrophy, mechanical stress responses, and recovery following myocardial injury.
Immunostaining using MYLK3 antibody reveals strong cytoplasmic localization in cardiac muscle fibers, particularly in ventricular tissue. The antibody is widely applied to research focused on cardiac muscle contractility, signal transduction, and kinase-mediated phosphorylation events. MYLK3 antibody from NSJ Bioreagents provides a reliable tool for investigating cardiac-specific myosin regulation, sarcomere dynamics, and disease mechanisms associated with cardiomyopathies. -
UniProt
- Q32MK0
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途径
- Regulation of Muscle Cell Differentiation
抗原
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