TFEC 抗体 (AA 27-311)
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Quick Overview for TFEC 抗体 (AA 27-311) (ABIN7873111)
抗原
See all TFEC 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 27-311
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原理
- TFEC Antibody / Transcription factor EC
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human TFEC recombinant protein (Position: Q27-D311) was used as the immunogen for the TFEC antibody.
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亚型
- IgG
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anti-Transcription Factor EC (TFEC) (AA 1-89) antibody
TFEC 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 4F11 unconjugated
anti-Transcription Factor EC (TFEC) (Internal Region) antibodyVerified TFEC 适用: 人, 小鼠 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Transcription Factor EC (TFEC) (N-Term) antibodyTFEC 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor EC (TFEC) (AA 179-205) antibodyTFEC 适用: 人 WB 宿主: 兔 Polyclonal RB40605 unconjugated
anti-Transcription Factor EC (TFEC) (N-Term) antibodyVerified TFEC 适用: 人 ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-Transcription Factor EC (TFEC) (AA 1-197) antibodyTFEC 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor EC (TFEC) (AA 71-120) antibodyTFEC 适用: 人, 大鼠, 犬, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the TFEC antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TFEC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- TFEC (Transcription Factor EC (TFEC))
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别名
- TFEC
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背景
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TFEC antibody detects Transcription factor EC, a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor that regulates gene expression in macrophages, osteoclasts, and melanocytes. TFEC belongs to the MiT/TFE family, which includes MITF, TFE3, and TFEB, all of which coordinate transcriptional responses to nutrient availability, lysosomal function, and immune signaling. The TFEC antibody is widely used in transcriptional regulation, macrophage differentiation, and cancer research.
TFEC is encoded by the TFEC gene located on human chromosome 7q31.2. The protein is approximately 38 kilodaltons and contains a conserved DNA-binding bHLH-LZ domain that mediates binding to E-box sequences in gene promoters. TFEC forms homodimers or heterodimers with other MiT family members, allowing versatile regulation of target gene networks. It localizes to the nucleus and is activated by phosphorylation under stress or inflammatory conditions.
The TFEC antibody detects a 38 kilodalton protein by western blot and demonstrates nuclear staining in macrophages and osteoclast precursors. TFEC regulates transcription of lysosomal and autophagy-related genes, participating in cellular adaptation to nutrient deprivation. It also modulates inflammatory gene expression through cooperation with PU.1 and other macrophage-specific transcription factors, promoting cytokine and chemokine production.
In melanocytes, TFEC contributes to pigment cell differentiation and interacts with MITF to balance melanogenic gene expression. Dysregulation of TFEC has been implicated in cancer, where fusion genes involving TFEC drive aberrant transcriptional programs. Altered expression is also associated with inflammatory and metabolic disorders due to disrupted lysosomal signaling and autophagic control.
TFEC integrates environmental and metabolic signals to maintain homeostasis in immune and pigment cells. NSJ Bioreagents provides a validated TFEC antibody optimized for western blot, immunofluorescence, and transcriptional studies, supporting detailed analysis of gene regulation, macrophage biology, and MiT/TFE family signaling. -
UniProt
- O14948
抗原
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