LGR5 抗体 (AA 26-907)
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Quick Overview for LGR5 抗体 (AA 26-907) (ABIN7873020)
抗原
See all LGR5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 26-907
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原理
- LGR5 Antibody / Leucine-rich repeat-containing G-protein coupled receptor 5
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human LGR5 recombinant protein (Position: R26-L907) was used as the immunogen for the LGR5 antibody.
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亚型
- IgG
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anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 810-907) antibody
LGR5 适用: 人, 小鼠 WB, FACS, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 443-473) antibodyLGR5 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB14555 unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 22-178) antibodyLGR5 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2B5B9 unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 250-550) antibodyLGR5 适用: 人, 小鼠 WB, FACS, IHC, IF, IP 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 443-473) antibodyLGR5 适用: 人 WB, FACS, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719) antibodyLGR5 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (Cytoplasmic Domain) antibodyLGR5 适用: 人 IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) antibodyLGR5 适用: 人 WB, FACS, ELISA 宿主: 兔 Monoclonal 4H8 unconjugated Recombinant Antibody
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 241-352) antibodyLGR5 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (N-Term) antibodyLGR5 适用: 人, Bat, 猴 IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the LGR5 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the LGR5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))
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别名
- LGR5
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背景
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LGR5 antibody detects Leucine-rich repeat-containing G-protein coupled receptor 5, a stem-cell marker and receptor for R-spondin proteins that potentiate Wnt/beta-catenin signaling. LGR5 is essential for maintaining self-renewing adult stem cell populations in the intestine, hair follicle, and stomach. The LGR5 antibody is used in stem-cell biology, oncology, and regenerative medicine to identify proliferative stem cell compartments.
LGR5 is encoded by the LGR5 gene on human chromosome 12q21.1. The receptor is approximately 907 amino acids long, with seven transmembrane domains typical of GPCRs and a large extracellular region containing leucine-rich repeats that mediate ligand binding. Upon R-spondin binding, LGR5 interacts with Frizzled and LRP5/6 receptors, amplifying Wnt signaling to sustain stem-cell proliferation.
The LGR5 antibody detects a 100-120 kilodalton protein by western blot and exhibits membrane and cytoplasmic localization in proliferative epithelial zones. In the intestine, LGR5 marks crypt base columnar cells capable of regenerating the entire epithelium. In the hair follicle, it labels bulge stem cells responsible for hair cycle renewal.
LGR5 expression is tightly regulated by Wnt activity and is a key marker for cancer stem cells in colorectal and gastric carcinomas. Overexpression promotes tumor initiation and therapy resistance, while knockdown suppresses proliferation and restores differentiation. The receptor modulates beta-catenin stabilization, transcription of target genes, and maintenance of undifferentiated states.
Because of its role in tissue regeneration and cancer stemness, LGR5 is an important biomarker for stem-cell tracking and targeted therapy development. NSJ Bioreagents provides a validated LGR5 antibody optimized for its applications, enabling precise identification of Wnt-responsive stem cell populations and cancer progenitors. -
UniProt
- O75473
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途径
- WNT signaling
抗原
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