ARHGEF7 抗体 (AA 25-637)
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北京 101111
Quick Overview for ARHGEF7 抗体 (AA 25-637) (ABIN7872887)
抗原
See all ARHGEF7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 25-637
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原理
- ARHGEF7 Antibody / Beta-Pix / Rho guanine nucleotide exchange factor 7
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human ARHGEF7 recombinant protein (Position: S25-N637) was used as the immunogen for the ARHGEF7 antibody.
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亚型
- IgG
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anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) antibody
ARHGEF7 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 1-250) antibodyARHGEF7 适用: 人 ELISA, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) antibodyARHGEF7 适用: 人, 大鼠, 小鼠 WB, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 1-188) antibodyARHGEF7 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 654-688) antibodyARHGEF7 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB56222 unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 102-190) antibodyARHGEF7 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 1-646) antibodyARHGEF7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) antibody (Biotin)ARHGEF7 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) antibody (FITC)ARHGEF7 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) antibody (HRP)ARHGEF7 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
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应用备注
- Optimal dilution of the ARHGEF7 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the ARHGEF7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))
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别名
- ARHGEF7
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背景
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ARHGEF7 antibody detects Rho guanine nucleotide exchange factor 7, a cytoskeletal signaling protein that regulates Rho GTPase activation, cell adhesion, and migration. Encoded by the ARHGEF7 gene on chromosome 13q34, this protein-also known as Beta-PIX (PAK-interacting exchange factor beta)-functions as a guanine nucleotide exchange factor (GEF) for the small GTPases Rac1 and Cdc42. Through these interactions, ARHGEF7 controls actin cytoskeleton remodeling, focal adhesion turnover, and lamellipodia formation essential for cell motility and morphology.
Structurally, ARHGEF7 contains several modular domains, including an SH3 domain that interacts with PAK kinases, a DH (Dbl homology) domain responsible for nucleotide exchange activity, and a PH (pleckstrin homology) domain that binds phosphoinositides for membrane targeting. These domains enable ARHGEF7 to act as a scaffold connecting small GTPases, kinases, and adhesion proteins such as GIT1 and paxillin. It localizes predominantly at focal adhesions and synaptic sites, integrating extracellular signals with intracellular cytoskeletal responses.
The ARHGEF7 antibody is widely used in cell signaling, cancer biology, and neurobiology research to study cytoskeletal organization, cell motility, and synaptic regulation. Western blot analysis detects a 76 kilodalton band corresponding to ARHGEF7, while immunofluorescence reveals punctate staining along the cell cortex, focal adhesions, and neuronal synapses. This antibody supports exploration of actin dynamics and Rho GTPase signaling in both normal and pathological contexts.
Functionally, ARHGEF7 interacts with p21-activated kinases (PAKs) to form complexes that control Rac1/Cdc42 activation and downstream signaling to the MAPK and JNK pathways. In neurons, it contributes to dendritic spine formation and synaptic plasticity by linking postsynaptic density proteins to actin assembly machinery. Dysregulation of ARHGEF7 has been associated with cancer metastasis, psychiatric disorders, and developmental defects due to altered Rho signaling. The ARHGEF7 antibody provides a dependable tool for dissecting these pathways and quantifying GEF expression in various models. NSJ Bioreagents validates this antibody for its applications, ensuring robust performance for cytoskeletal and signaling studies. -
UniProt
- Q14155
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途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
抗原
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