GCLC 抗体 (AA 24-388)
Our Local Distributor
北京 101111
Quick Overview for GCLC 抗体 (AA 24-388) (ABIN7872689)
抗原
See all GCLC 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 24-388
-
原理
- GCLC Antibody / Glutamate-cysteine ligase catalytic subunit
-
纯化方法
- Immunogen affinity purified
-
免疫原
- E.coli-derived human GCLC recombinant protein (Position: R24-H388) was used as the immunogen for the GCLC antibody.
-
亚型
- IgG
-
-
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 528-637) antibody
GCLC 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3H1 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 437-637) antibodyGCLC 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) antibodyKD Validated GCLC 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB4860 unconjugated Recombinant Antibody
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30), (N-Term) antibodyGCLC 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB31687 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 豚鼠, 犬, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30) antibodyGCLC 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-252) antibodyGCLC 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) antibodyGCLC 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibodyGCLC 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal dilution of the GCLC antibody should be determined by the researcher.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- After reconstitution, the GCLC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
-
-
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
-
别名
- GCLC
-
背景
-
GCLC antibody detects Glutamate-cysteine ligase catalytic subunit, a key enzyme encoded by the GCLC gene located on chromosome 6p12.1. GCLC catalyzes the first and rate-limiting step in glutathione (GSH) biosynthesis, forming gamma-glutamylcysteine from glutamate and cysteine. This enzyme plays an essential role in maintaining redox homeostasis, detoxifying reactive oxygen species (ROS), and protecting cells from oxidative stress. GCLC is expressed ubiquitously, with highest levels in liver, kidney, and lung, where glutathione metabolism is most active.
Structurally, GCLC is a 73 kDa cytosolic enzyme that functions as the catalytic component of the heterodimeric glutamate-cysteine ligase complex, together with the modifier subunit GCLM. It contains ATP-binding and substrate-recognition domains that mediate the ligation reaction. GCLC belongs to the ATP-dependent ligase family and serves as the principal control point in GSH biosynthesis. Co-localization studies show cytoplasmic distribution in metabolically active tissues, aligning with its antioxidant role.
Functionally, GCLC maintains cellular antioxidant defenses by providing the precursor for glutathione synthesis. Glutathione serves as a cofactor for numerous detoxification enzymes, including glutathione peroxidases and glutathione S-transferases. Through GSH production, GCLC supports redox regulation, protein thiol homeostasis, and protection against electrophilic stress. In immune cells, GCLC-derived glutathione regulates T-cell activation and macrophage inflammatory responses. Known substrates include L-glutamate, L-cysteine, and ATP.
Deficiency or dysregulation of GCLC results in decreased glutathione levels, leading to oxidative stress, hemolytic anemia, and liver dysfunction. Mutations in GCLC are associated with glutathione synthetase deficiency and neurological disorders linked to oxidative damage. Overexpression is observed in certain cancers, conferring chemoresistance through enhanced antioxidant capacity. Pathway associations include glutathione metabolism, oxidative stress response, and xenobiotic detoxification. During development, GCLC supports organogenesis by protecting proliferating cells from oxidative injury.
The GCLC antibody from NSJ Bioreagents is an excellent reagent for studies involving redox biology, detoxification mechanisms, and glutathione metabolism. -
UniProt
- P48506
-
途径
- Cell RedoxHomeostasis
抗原
-
