MPG 抗体 (AA 20-298)
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Quick Overview for MPG 抗体 (AA 20-298) (ABIN7871858)
抗原
See all MPG 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 20-298
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原理
- DNA-3-methyladenine glycosylase Antibody / MPG / AAG
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纯化方法
- Antigen affinity purified
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免疫原
- Recombinant human protein (amino acids Q20-A298) was used as the immunogen for the DNA-3-methyladenine glycosylase antibody.
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亚型
- IgG
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应用备注
- Optimal dilution of the DNA-3-methyladenine glycosylase antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the DNA-3-methyladenine glycosylase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- MPG (N-Methylpurine-DNA Glycosylase (MPG))
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别名
- DNA-3-methyladenine glycosylase
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背景
- MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG and APNG is a protein expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line(HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo,Hang et al.(1997)biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.
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UniProt
- P29372
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途径
- DNA Damage Repair
抗原
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