EFS 抗体 (AA 183-553)
The 兔 多克隆 anti-EFS antibody (ABIN7871470) specifically detects EFS in WB, ELISA, IP 和 FACS.
The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7871470
发货至:
中国
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Contact Info
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中国
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Quick Overview for EFS 抗体 (AA 183-553) (ABIN7871470)
抗原
See all EFS 抗体
EFS
(Embryonal Fyn-Associated Substrate (EFS))
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This EFS antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
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抗原表位
- AA 183-553
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原理
- EFS Antibody / Embryonal Fyn-associated substrate
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human EFS recombinant protein (Position: E183-T553) was used as the immunogen for the EFS antibody.
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亚型
- IgG
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anti-Embryonal Fyn-Associated Substrate (EFS) (AA 367-399) antibody
EFS 适用: 人 WB 宿主: 兔 Polyclonal RB54269 unconjugated
anti-Embryonal Fyn-Associated Substrate (EFS) (AA 1-468) antibodyEFS 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal dilution of the EFS antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the EFS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- EFS (Embryonal Fyn-Associated Substrate (EFS))
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别名
- EFS
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背景
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EFS antibody detects Embryonal Fyn-associated substrate, encoded by the EFS gene on chromosome 14q11.2. EFS antibody is commonly applied in studies of cytoskeletal organization, adhesion, and signaling. EFS belongs to the CAS (Crk-associated substrate) family of adaptor proteins, which includes BCAR1 (p130Cas) and NEDD9. It is predominantly expressed in embryonic tissues, endothelial cells, and hematopoietic cells, where it mediates signaling downstream of integrins, growth factors, and tyrosine kinases. Its discovery as a substrate of Fyn kinase highlighted its role in Src family kinase signaling networks.
Structurally, EFS contains an N-terminal SH3 domain, a substrate domain with multiple tyrosine phosphorylation sites, and a C-terminal focal adhesion targeting region. These domains allow EFS to interact with integrins, kinases, and actin cytoskeleton regulators. Phosphorylation of tyrosine residues by Src family kinases creates binding sites for SH2-containing adaptors such as Crk, linking EFS to downstream signaling cascades controlling adhesion and migration.
Functionally, EFS regulates integrin-mediated adhesion, migration, and cytoskeletal remodeling. It acts as a molecular scaffold at focal adhesions, coupling mechanical signals to intracellular pathways. EFS also participates in growth factor signaling, coordinating responses to PDGF, EGF, and VEGF. Knockdown of EFS reduces migration and disrupts adhesion dynamics, underscoring its importance in cell motility. Researchers employ EFS antibody to investigate focal adhesion signaling, cytoskeletal dynamics, and integrin biology.
Clinically, EFS has been linked to cancer, where altered expression contributes to invasion and metastasis. Its phosphorylation status serves as a marker of Src family kinase activity. In leukemia, EFS participates in abnormal signaling pathways that promote proliferation. EFS has also been studied in cardiovascular biology, where it regulates endothelial cell adhesion and angiogenesis. NSJ Bioreagents offers EFS antibody for use in oncology, vascular biology, and cell signaling research.
Experimentally, EFS antibody is applied in western blotting to detect the ~78 kDa protein, in immunohistochemistry to evaluate tissue distribution, and in immunofluorescence microscopy to visualize focal adhesion localization. Immunoprecipitation with EFS antibody enables analysis of phosphorylation status and identification of binding partners. -
UniProt
- O43281
抗原
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