NFIA 抗体 (AA 172-233)
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北京 101111
Quick Overview for NFIA 抗体 (AA 172-233) (ABIN7871264)
抗原
See all NFIA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 172-233
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原理
- NFIA Antibody / Nuclear factor 1 A-type
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NFIA recombinant protein (Position: V172-Q233) was used as the immunogen for the NFIA antibody.
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亚型
- IgG
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anti-Nuclear Factor I/A (NFIA) (AA 1-498) antibody
NFIA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E11 unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-330) antibodyNFIA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-224), (Middle Region) antibodyNFIA 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 348-397) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, Pig, 豚鼠, 小鸡, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-498) antibodyNFIA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 384-418), (C-Term) antibodyNFIA 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB51284 unconjugated
anti-Nuclear Factor I/A (NFIA) (C-Term) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (Middle Region) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 犬, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-143) antibodyNFIA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-330) antibodyNFIA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NFIA antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NFIA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NFIA (Nuclear Factor I/A (NFIA))
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别名
- NFIA
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背景
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NFIA antibody detects Nuclear factor 1 A-type, a member of the NFI transcription factor family involved in gene regulation during development, differentiation, and glial cell maturation. NFIA functions as both an activator and repressor of transcription and binds to the palindromic NFI recognition site present in numerous promoters. It plays critical roles in the development of the central nervous system, particularly in astrocyte differentiation, corpus callosum formation, and regulation of glial-specific gene expression. The NFIA antibody is a widely used tool for exploring transcriptional mechanisms that control neural development and glial identity.
NFIA is encoded by the NFIA gene located on human chromosome 1p31.3. The protein contains a conserved N-terminal DNA-binding domain responsible for dimerization and sequence-specific binding, along with a C-terminal transactivation/repression region that interacts with other transcriptional regulators. NFIA forms homo- and heterodimers with other NFI family members (NFIB, NFIC, NFIX), allowing combinatorial control of target genes. Studies in model organisms have shown that loss of NFIA disrupts gliogenesis, leading to abnormal brain structure and impaired glial differentiation.
Expression profiling reveals that NFIA is abundant in glial progenitor cells and astrocytes, while its expression declines as neurons mature. Western blot analysis using NFIA antibody typically identifies a band near 54-60 kDa (unphosphorylated form), corresponding to the full-length transcription factor. Immunohistochemistry reveals strong nuclear localization in developing brain regions, spinal cord, and other tissues undergoing differentiation. NFIA cooperates with SOX9, STAT3, and other transcriptional factors to regulate astrocytic gene programs, such as those encoding GFAP and S100B.
In addition to its role in neurodevelopment, NFIA participates in the regulation of kidney morphogenesis, adipogenesis, and hematopoietic stem cell maintenance. Dysregulation or mutation of NFIA has been linked to developmental delay, macrocephaly, and agenesis of the corpus callosum in human syndromic cases. NSJ Bioreagents provides a validated NFIA antibody optimized for western blot, immunohistochemistry, and chromatin studies, making it a key reagent for dissecting transcriptional networks in development and disease. -
UniProt
- Q12857
抗原
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