Prohibitin 抗体 (AA 167-261)
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Quick Overview for Prohibitin 抗体 (AA 167-261) (ABIN7871167)
抗原
See all Prohibitin (PHB) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 167-261
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原理
- Prohibitin Antibody / PHB (azide and preservative free)
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纯化方法
- Protein G affinity chromatography
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免疫原
- A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.
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亚型
- IgG2a, kappa
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应用备注
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Optimal dilution of the Prohibitin antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Prohibitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Prohibitin (PHB)
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别名
- Prohibitin
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背景
- This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
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UniProt
- P35232
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
抗原
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