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Prohibitin 抗体 (AA 167-261)

The 小鼠 单克隆 anti-Prohibitin antibody (Clone PHB-3230) (ABIN7871167) specifically detects Prohibitin in WB 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7871167
发货至: 中国
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Quick Overview for Prohibitin 抗体 (AA 167-261) (ABIN7871167)

抗原

See all Prohibitin (PHB) 抗体
Prohibitin (PHB)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Prohibitin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

质量等级

Carrier-free

克隆位点

PHB-3230
  • 抗原表位

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    AA 167-261

    原理

    Prohibitin Antibody / PHB (azide and preservative free)

    纯化方法

    Protein G affinity chromatography

    免疫原

    A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.

    亚型

    IgG2a, kappa
  • 应用备注

    Optimal dilution of the Prohibitin antibody should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Prohibitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    Prohibitin (PHB)

    别名

    Prohibitin

    背景

    This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

    UniProt

    P35232

    途径

    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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