SUN5 抗体 (AA 16-341)
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Quick Overview for SUN5 抗体 (AA 16-341) (ABIN7871069)
抗原
See all SUN5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 16-341
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原理
- SUN5 Antibody / Sad1 and UNC84 domain-containing protein 5
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SUN5 recombinant protein (Position: E16-K341) was used as the immunogen for the SUN5 antibody.
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亚型
- IgG
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anti-Sad1 and UNC84 Domain Containing 5 (SUN5) (AA 161-275) antibody
SUN5 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sad1 and UNC84 Domain Containing 5 (SUN5) antibodySUN5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sad1 and UNC84 Domain Containing 5 (SUN5) (AA 1-379) antibodySUN5 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Sad1 and UNC84 Domain Containing 5 (SUN5) (N-Term) antibodySUN5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sad1 and UNC84 Domain Containing 5 (SUN5) (N-Term) antibodySUN5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sad1 and UNC84 Domain Containing 5 (SUN5) (N-Term) antibodySUN5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the SUN5 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SUN5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))
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别名
- SUN5
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背景
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SUN5 antibody detects Sad1 and UNC84 domain-containing protein 5, a nuclear envelope protein that anchors the nucleus to the cytoskeleton during spermatogenesis. The UniProt recommended name is Sad1 and UNC84 domain-containing protein 5 (SUN5). This transmembrane protein is part of the linker of nucleoskeleton and cytoskeleton (LINC) complex, which connects nuclear components to cytoskeletal filaments, ensuring nuclear positioning and structural integrity.
Functionally, SUN5 antibody identifies a 379-amino-acid protein localized to the inner nuclear membrane, where it interacts with outer membrane proteins such as Nesprins through its conserved SUN domain. SUN5 is particularly enriched in spermatids, where it mediates head-to-tail coupling during sperm maturation. Loss of SUN5 disrupts this linkage, leading to acephalic spermatozoa and infertility.
The SUN5 gene is located on chromosome 20q11.22 and is predominantly expressed in testis, with lower expression in other tissues. Its testis-specific expression pattern underscores its specialized function in spermiogenesis. SUN5 contributes to nuclear shaping, centrosome attachment, and flagellar organization through mechanical coupling between the nucleus and cytoskeleton.
Pathologically, mutations in SUN5 are associated with acephalic spermatozoa syndrome and male infertility due to defective sperm head-tail junction formation. Research using SUN5 antibody supports studies in reproductive biology, nuclear envelope architecture, and cytoskeletal interaction mechanisms.
SUN5 antibody is validated for use in western blotting, immunofluorescence, and immunohistochemistry to study LINC complex components and spermatid structure. NSJ Bioreagents offers SUN5 antibody reagents optimized for cell biology and reproductive system research.
Structurally, SUN5 contains a coiled-coil region and a conserved C-terminal SUN domain that mediates interaction with KASH domain proteins across the nuclear envelope. This antibody helps elucidate SUN5's molecular function in nuclear-cytoskeletal attachment and fertility-related disorders. -
UniProt
- Q8TC36
抗原
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