PLS1 抗体 (AA 14-581)
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北京 101111
Quick Overview for PLS1 抗体 (AA 14-581) (ABIN7870651)
抗原
See all PLS1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 14-581
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原理
- PLS1 Antibody / Plastin-1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PLS1 recombinant protein (Position: E14-L581) was used as the immunogen for the PLS1 antibody.
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亚型
- IgG
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anti-Plastin 1 (PLS1) (AA 1-143) antibody
PLS1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Plastin 1 (PLS1) (AA 1-102) antibodyPLS1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3G10 unconjugated
anti-Plastin 1 (PLS1) antibodyPLS1 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Plastin 1 (PLS1) (AA 1-110) antibodyPLS1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Plastin 1 (PLS1) (AA 550-579), (C-Term) antibodyPLS1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32565 unconjugated
anti-Plastin 1 (PLS1) (AA 71-120) antibodyPLS1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PLS1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PLS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PLS1 (Plastin 1 (PLS1))
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别名
- PLS1
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背景
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PLS1 antibody detects Plastin-1, an actin-bundling protein that stabilizes and crosslinks actin filaments in epithelial and sensory cells. Encoded by the PLS1 gene on chromosome 3q23, this protein belongs to the plastin family of actin-bundling proteins, which includes isoforms expressed in hematopoietic, ubiquitous, and epithelial tissues. Plastin-1 plays an essential structural role in maintaining brush border microvilli in the intestinal epithelium and stereocilia in the inner ear, contributing to cellular rigidity and signal transduction.
Structurally, Plastin-1 is a 630-amino-acid cytoskeletal protein of approximately 68 kilodaltons containing two tandem calponin homology (CH) domains responsible for actin binding and two EF-hand calcium-binding motifs that regulate its activity. The protein localizes predominantly to actin-rich structures such as microvilli and adherens junctions, where it bundles filamentous actin into parallel arrays that reinforce cell shape and polarity.
The PLS1 antibody is widely used in cell biology, auditory physiology, and epithelial research to study cytoskeletal architecture, microvillar dynamics, and actin filament regulation. Western blot analysis detects a 68 kilodalton band corresponding to Plastin-1, while immunofluorescence demonstrates strong staining along apical membranes in epithelial cells. This antibody provides a reliable tool for investigating actin organization and microvillar maintenance.
Functionally, Plastin-1 is critical for maintaining the structural stability of brush border and sensory epithelia by linking actin filaments into tightly packed bundles. Mutations in PLS1 cause autosomal recessive nonsyndromic hearing loss due to disruption of stereocilia structure and function in cochlear hair cells. In intestinal cells, PLS1 supports nutrient absorption and epithelial resilience under mechanical stress. The PLS1 antibody supports studies of cytoskeletal integrity, epithelial morphogenesis, and hereditary hearing loss. NSJ Bioreagents validates this antibody for its applications, ensuring accurate detection of Plastin-1 in cytoskeletal research. -
UniProt
- Q14651
抗原
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