Histone H2A 抗体 (AA 115-143)
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Quick Overview for Histone H2A 抗体 (AA 115-143) (ABIN7870200)
抗原
See all Histone H2A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 115-143
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原理
- Histone H2AX Antibody / H2AFX
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纯化方法
- Protein G affinity
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免疫原
- A portion of amino acids 115-143 from the human protein was used as the immunogen for the Histone H2AX antibody.
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亚型
- IgG1
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应用备注
- The stated application concentrations are suggested starting points. Titration of the Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the Histone H2AX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- Histone H2A
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背景
- Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
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UniProt
- P16104
抗原
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