LIMS1 抗体 (AA 11-172)
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北京 101111
Quick Overview for LIMS1 抗体 (AA 11-172) (ABIN7870068)
抗原
See all LIMS1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 11-172
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原理
- LIMS1 Antibody / LIM and senescent cell antigen-like-containing domain protein 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human LIMS1 recombinant protein (Position: E11-A172) was used as the immunogen for the LIMS1 antibody.
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亚型
- IgG
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anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibody
LIMS1 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5G7 unconjugated
anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) (AA 1-325) antibodyLIMS1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibodyLIMS1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) (AA 68-303) antibodyLIMS1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) (AA 28-54) antibodyLIMS1 适用: 人 WB 宿主: 兔 Polyclonal RB38877 unconjugated
anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibodyLIMS1 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the LIMS1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the LIMS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))
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别名
- LIMS1
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背景
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LIMS1 antibody detects LIM and senescent cell antigen-like-containing domain protein 1, encoded by the LIMS1 gene on chromosome 2q12.3. LIMS1 antibody is widely applied in studies of integrin signaling, cell adhesion, and cancer biology. LIMS1, also known as PINCH1, is a LIM domain-containing adaptor protein that localizes to focal adhesions, where it connects integrins with the actin cytoskeleton and signaling pathways. It plays a central role in cell-matrix interactions and regulation of survival, proliferation, and migration.
Structurally, LIMS1 is a ~37 kDa protein composed of five LIM domains, which mediate protein-protein interactions. Through these domains, LIMS1 interacts with integrin-linked kinase (ILK) and parvin to form the IPP complex, a key signaling hub at focal adhesions. This complex links extracellular matrix adhesion with intracellular signaling cascades.
Functionally, LIMS1 stabilizes focal adhesions and promotes integrin-mediated signaling. It regulates pathways including PI3K-Akt, MAPK, and Rho GTPase signaling, influencing survival, motility, and cytoskeletal organization. Knockout models demonstrate that LIMS1 is essential for embryonic development and cell viability. Researchers use LIMS1 antibody to investigate adhesion signaling, integrin function, and tumor progression.
Clinically, LIMS1 is associated with cancer, where its overexpression promotes invasion and metastasis by enhancing integrin signaling. It also influences therapy resistance in multiple tumor types. Genetic studies have linked LIMS1 variants to immune function and kidney transplantation outcomes, suggesting roles beyond adhesion. NSJ Bioreagents supplies LIMS1 antibody for use in adhesion biology, immunology, and oncology.
Experimentally, LIMS1 antibody is applied in western blotting to detect the ~37 kDa protein, in immunofluorescence to visualize focal adhesion localization, and in immunohistochemistry to assess tumor expression. Co-immunoprecipitation with LIMS1 antibody identifies ILK, parvin, and integrin-binding partners. -
UniProt
- P48059
抗原
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