LIG4 抗体 (AA 1-876)
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Quick Overview for LIG4 抗体 (AA 1-876) (ABIN7869599)
抗原
See all LIG4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-876
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原理
- LIG4 Antibody / DNA Ligase 4
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876) was used as the immunogen for the LIG4 antibody.
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亚型
- IgG
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anti-Ligase IV, DNA, ATP-Dependent (LIG4) antibody
KD Validated LIG4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2515 unconjugated Recombinant Antibody
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (C-Term) antibodyLIG4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 612-911) antibodyLIG4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (N-Term) antibodyLIG4 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 802-911) antibodyLIG4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D2 unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (N-Term) antibodyLIG4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 275-324) antibodyLIG4 适用: 人, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) antibodyKO Validated LIG4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (Center) antibodyLIG4 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 1-911) antibodyLIG4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal dilution of the LIG4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the LIG4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))
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别名
- LIG4
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背景
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LIG4 antibody detects DNA ligase 4, an essential enzyme that catalyzes the final ligation step in non-homologous end joining (NHEJ) DNA repair. The UniProt recommended name is DNA ligase 4 (LIG4). This enzyme joins DNA double-strand breaks generated during recombination, replication stress, or DNA damage, preserving genome stability.
Functionally, LIG4 antibody identifies a 911-amino-acid ATP-dependent DNA ligase that seals nicks in double-stranded DNA by catalyzing phosphodiester bond formation. LIG4 operates in complex with XRCC4 and XLF/Cernunnos to align DNA ends and complete repair during NHEJ. It also plays a critical role in V(D)J recombination in lymphocytes, supporting immune diversity.
The LIG4 gene is located on chromosome 13q33.3 and is ubiquitously expressed in proliferating tissues. Its expression is highest in immune cells, where DNA recombination occurs frequently. Proper function of LIG4 ensures efficient DNA repair and prevents chromosomal translocations and mutagenesis.
Pathologically, mutations in LIG4 cause Ligase IV syndrome, characterized by microcephaly, developmental delay, immunodeficiency, and radiosensitivity. Deficiency in LIG4 impairs DNA double-strand break repair, leading to genomic instability and increased cancer susceptibility. Research using LIG4 antibody aids in studies of DNA repair, recombination, and genomic maintenance.
LIG4 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect ligase enzymes in DNA damage response pathways. NSJ Bioreagents provides LIG4 antibody reagents optimized for DNA repair, oncology, and immunology research.
Structurally, DNA ligase 4 contains an N-terminal DNA-binding domain, an adenylation domain that binds AMP, and a C-terminal XRCC4-interacting region necessary for NHEJ complex formation. This antibody enables detailed analysis of LIG4's enzymatic activity in genome integrity and repair pathways. -
UniProt
- P49917
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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