Esterase D 抗体 (AA 1-282)
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Quick Overview for Esterase D 抗体 (AA 1-282) (ABIN7869044)
抗原
See all Esterase D (ESD) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-282
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原理
- ESD Antibody / Esterase D
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human ESD recombinant protein (Position: M1-A282) was used as the immunogen for the ESD antibody.
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亚型
- IgG
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anti-Esterase D (ESD) antibody
ESD 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Esterase D (ESD) (AA 183-281) antibodyESD 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E1 unconjugated
anti-Esterase D (ESD) (AA 1-282) antibodyESD 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Esterase D (ESD) (AA 1-282) antibodyESD 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Esterase D (ESD) (AA 68-102) antibodyESD 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB47739 unconjugated
anti-Esterase D (ESD) (N-Term) antibodyESD 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Esterase D (ESD) (AA 1-282) antibodyESD 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Esterase D (ESD) (AA 2-282) antibodyESD 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Esterase D (ESD) (N-Term) antibodyESD 适用: 人, Cow, 犬, 马, Pig, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Esterase D (ESD) (N-Term) antibodyESD 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, Pig, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the ESD antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the ESD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Esterase D (ESD)
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别名
- ESD
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背景
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ESD antibody detects Esterase D, encoded by the ESD gene on chromosome 13q14.11. ESD antibody is used to study this cytosolic serine hydrolase involved in detoxification and xenobiotic metabolism. ESD was originally characterized as a polymorphic enzyme used in forensic medicine for paternity testing and blood typing. Beyond this historical role, Esterase D has biological functions in processing of ester-containing molecules, detoxification of aldehydes, and metabolism of drugs and environmental chemicals. It is expressed in liver, kidney, lung, and erythrocytes, reflecting its general detoxification role.
Structurally, Esterase D is a 282 amino acid protein with an alpha/beta hydrolase fold. It contains the conserved catalytic triad of serine, histidine, and aspartate typical of serine esterases. Its active site is adapted for hydrolyzing a wide range of small esters. Genetic polymorphisms in ESD lead to enzyme variants with distinct electrophoretic mobility, which historically formed the basis of forensic applications. The conserved structure supports its role as a versatile detoxifying enzyme.
Functionally, ESD hydrolyzes ester bonds in endogenous and exogenous compounds, contributing to clearance of aldehydes and drugs. It also participates in formaldehyde detoxification by metabolizing S-formylglutathione. Knockout or deficiency studies suggest that loss of Esterase D activity impairs detoxification pathways and increases susceptibility to oxidative stress. Researchers employ ESD antibody to study its enzymatic activity, tissue distribution, and regulation under toxicant exposure.
Clinically, ESD deficiency has been associated with retinoblastoma and other developmental disorders due to its chromosomal location near the RB1 tumor suppressor gene. Altered ESD activity is also reported in liver disease, hemolytic anemia, and metabolic disorders. As a polymorphic enzyme, Esterase D has served as a genetic marker in population genetics and forensic investigations. Its ability to detoxify aldehydes and esters makes it relevant to environmental health and pharmacology. NSJ Bioreagents supplies ESD antibody for research into metabolism, toxicology, and disease associations.
Experimentally, ESD antibody is used in western blotting to detect the ~33 kDa protein, in enzymatic assays to measure esterase activity, and in immunohistochemistry to study tissue expression. Immunoprecipitation with ESD antibody allows analysis of its enzyme complexes and potential regulatory partners. These applications provide tools for dissecting detoxification pathways and oxidative stress regulation. -
UniProt
- P10768
抗原
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