Phosducin 抗体 (AA 1-246)
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Quick Overview for Phosducin 抗体 (AA 1-246) (ABIN7868921)
抗原
See all Phosducin (PDC) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-246
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原理
- PDC Antibody / Phosducin
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PDC recombinant protein (Position: M1-E246) was used as the immunogen for the PDC antibody.
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亚型
- IgG
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anti-Phosducin (PDC) antibody
PDC 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosducin (PDC) (AA 1-246) antibodyPDC 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosducin (PDC) (AA 1-194) antibodyPDC 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Phosducin (PDC) (AA 59-88) antibodyPDC 适用: 人 WB 宿主: 兔 Polyclonal RB31115 unconjugated
anti-Phosducin (PDC) antibodyPDC 适用: Arabidopsis thaliana, Oryza sativa, Zymomonas mobilis WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PDC antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PDC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Phosducin (PDC)
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别名
- PDC
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背景
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PDC antibody detects Phosducin, a phosphoprotein that modulates G-protein signaling in photoreceptor cells and other neurons. The UniProt recommended name is Phosducin (PDC), an abundant cytosolic protein expressed primarily in the retina. Phosducin regulates heterotrimeric G-protein signaling by binding the beta-gamma subunits (Gbetagamma) of transducin, thereby controlling phototransduction and other G-protein-mediated pathways.
Functionally, PDC antibody identifies a 28 kDa protein that plays a central role in the visual signal transduction cascade. In dark-adapted photoreceptors, phosducin forms a complex with Gbetagamma subunits, preventing reassociation with the alpha subunit and thereby maintaining the inactive G-protein state. Upon light stimulation, elevated cAMP and phosphorylation by protein kinase A (PKA) reduce its affinity for Gbetagamma, freeing these subunits to reassemble with transducin alpha and activate cGMP phosphodiesterase. This mechanism ensures the precise timing and sensitivity of visual signaling.
The PDC gene is located on chromosome 1q25.2 and encodes a protein consisting of a central G-protein beta-binding domain. Beyond the retina, phosducin is expressed in pineal gland, olfactory neurons, and parts of the brain, suggesting broader roles in neuromodulation. It participates in feedback control of G-protein signaling, potentially influencing synaptic plasticity and hormone release. Phosducin is evolutionarily conserved, with homologs present in multiple vertebrate species, highlighting its importance in sensory transduction and neural signaling.
PDC antibody is used in neurobiology and vision research to explore phototransduction mechanisms, G-protein signaling regulation, and phosphorylation dynamics. In rod cells, phosphorylation state changes of phosducin reflect illumination conditions and photoreceptor adaptation. Studies also show that phosducin participates in stress responses by modulating sympathetic nervous system activity, linking retinal signaling pathways with systemic physiological regulation.
Structurally, phosducin comprises a coiled-coil N-terminal region required for Gbetagamma binding and a C-terminal domain that interacts with chaperones and kinases. It is phosphorylated at multiple serine residues, including Ser54 and Ser73, which alter its subcellular distribution and binding capacity. These regulatory mechanisms fine-tune G-protein signaling under varying environmental and metabolic conditions. Mutations or dysregulation of PDC expression have been implicated in retinal degeneration and light adaptation defects.
NSJ Bioreagents offers PDC antibody reagents validated for research applications in vision, signal transduction, and G-protein biology. These antibodies are suited for applications used to study the localization and regulation of phosducin in photoreceptor and neuronal systems. -
UniProt
- P20941
抗原
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