UBE2F 抗体 (AA 1-185)
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Quick Overview for UBE2F 抗体 (AA 1-185) (ABIN7868319)
抗原
See all UBE2F 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-185
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原理
- UBE2F Antibody / NEDD8-conjugating enzyme E2 F
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human UBE2F recombinant protein (Position: M1-R185) was used as the immunogen for the UBE2F antibody.
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亚型
- IgG
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anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) antibody
UBE2F 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyKD Validated UBE2F 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB940 unconjugated Recombinant Antibody
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyUBE2F 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyUBE2F 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyVerified UBE2F 适用: 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (C-Term) antibodyVerified UBE2F 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) antibodyUBE2F 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 猴, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (C-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, 猴, Bat, Hamster, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (Middle Region) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the UBE2F antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the UBE2F antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
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别名
- UBE2F
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背景
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UBE2F antibody detects NEDD8-conjugating enzyme E2 F, an E2 ubiquitin-like conjugating enzyme that catalyzes neddylation of cullin proteins, regulating cullin-RING ligase (CRL) activity. The UniProt recommended name is NEDD8-conjugating enzyme E2 F (UBE2F). This enzyme cooperates with the NEDD8-activating enzyme (NAE1/UBA3) and specific E3 ligases to attach NEDD8 to target substrates, modulating protein degradation pathways.
Functionally, UBE2F antibody identifies a 185-amino-acid cytoplasmic enzyme that activates and transfers NEDD8 to cullin 5 and other CRL components. UBE2F works specifically with the E3 ligase RBX2 (RNF7), forming a selective neddylation module distinct from UBE2M (UBC12). Through this modification, UBE2F regulates CRL activity, affecting cell cycle progression, signal transduction, and stress responses.
The UBE2F gene is located on chromosome 5q31.2 and is expressed in multiple tissues, including testis, brain, and lung. Its expression is cell cycle-dependent and induced under oxidative stress. UBE2F ensures timely activation of CRLs, thereby influencing ubiquitin-mediated degradation of regulatory proteins such as p27 and HIF1A.
Pathologically, dysregulation of UBE2F contributes to cancer and oxidative stress-related disorders. Overexpression promotes degradation of tumor suppressors, while inhibition can stabilize proteins that restrain cell proliferation. UBE2F has emerged as a therapeutic target for modulating proteostasis and redox balance in malignant cells.
UBE2F antibody is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect neddylation components and study ubiquitin-like modification systems. NSJ Bioreagents provides validated UBE2F antibody reagents for investigating protein turnover, neddylation, and cellular signaling.
Structurally, UBE2F contains a conserved catalytic cysteine residue within its E2 core domain, forming a thioester bond with activated NEDD8. This structure enables transfer to substrate-bound E3 ligases. This antibody helps characterize UBE2F's mechanistic role in the neddylation cascade and its impact on CRL activity. -
UniProt
- Q969M7
抗原
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