IFT20 抗体 (AA 1-132)
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Quick Overview for IFT20 抗体 (AA 1-132) (ABIN7868098)
抗原
See all IFT20 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-132
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原理
- IFT20 Antibody / Intraflagellar transport protein 20 homolog
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human IFT20 recombinant protein (Position: M1-K132) was used as the immunogen for the IFT20 antibody.
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亚型
- IgG
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anti-Intraflagellar Transport 20 Homolog (IFT20) (AA 1-148) antibody
IFT20 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F3 unconjugated
anti-Intraflagellar Transport 20 Homolog (IFT20) antibodyIFT20 适用: 人, 小鼠, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intraflagellar Transport 20 Homolog (IFT20) (AA 1-132) antibodyIFT20 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intraflagellar Transport 20 Homolog (IFT20) (AA 44-71), (Center) antibodyIFT20 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB24809 unconjugated
anti-Intraflagellar Transport 20 Homolog (IFT20) (AA 1-148) antibodyIFT20 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal dilution of the IFT20 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the IFT20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- IFT20 (Intraflagellar Transport 20 Homolog (IFT20))
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别名
- IFT20
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背景
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IFT20 antibody detects Intraflagellar transport protein 20 homolog, a component of the intraflagellar transport (IFT) complex required for cilium assembly, maintenance, and cargo trafficking. The UniProt recommended name is Intraflagellar transport protein 20 homolog (IFT20). This small protein serves as a crucial adaptor that links vesicular trafficking machinery to the IFT complex, ensuring proper delivery of membrane and signaling components to the cilium.
Functionally, IFT20 antibody identifies a 132-amino-acid protein localized to the Golgi apparatus and the base of cilia. IFT20 associates with the IFT-B complex and interacts with proteins such as IFT57, IFT88, and GMAP210 to coordinate transport of ciliary cargo from the Golgi to the basal body. It plays essential roles in ciliogenesis, ciliary membrane protein sorting, and Hedgehog signaling. IFT20 functions both in ciliated cells, such as renal epithelial cells and photoreceptors, and in non-ciliated cells, where it contributes to vesicle trafficking and immune synapse formation.
The IFT20 gene is located on chromosome 17q25.3 and is ubiquitously expressed in tissues with active ciliary assembly, including kidney, lung, retina, and brain. Expression is dynamically regulated during cell cycle progression and development, reflecting its importance in signaling and organelle biogenesis.
Pathologically, mutations or loss of IFT20 disrupt cilium formation and function, leading to a spectrum of ciliopathies such as polycystic kidney disease, retinal degeneration, and developmental disorders. Altered expression may also affect immune function, as IFT20 participates in T-cell receptor recycling. Research using IFT20 antibody supports studies in cilia biology, vesicular trafficking, and developmental signaling pathways.
IFT20 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ciliary transport proteins. NSJ Bioreagents provides IFT20 antibody reagents optimized for studies in ciliogenesis, Golgi-cilium communication, and membrane trafficking.
Structurally, Intraflagellar transport protein 20 homolog is a small coiled-coil protein that integrates into the IFT-B complex. Its N-terminal region anchors it to the Golgi, while the C-terminal region mediates interactions with IFT components. This structural configuration enables IFT20 to couple vesicular transport and ciliary assembly. This antibody aids in investigating IFT20's role in cellular signaling, ciliogenesis, and trafficking fidelity. -
UniProt
- Q8IY31
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途径
- Hedgehog Signaling
抗原
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