IKK-alpha /IKK-beta 抗体 (pSer176, pSer180)
(2 references)-
- 抗原 See all IKK-alpha /IKK-beta products
- IKK-alpha /IKK-beta
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抗原表位
- pSer176, pSer180
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适用
- 小鼠, 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IKK-alpha /IKK-beta antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- These phosphorylation sites are homologous across the listed species.
- 交叉反应
- 人, 小鼠
- 预测反应
- Rat,Dog,Cow,Pig,Horse,Chicken
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of Ser176/Ser180
- 亚型
- IgG
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anti-IKK-alpha /IKK-beta (pSer180), (pSer181) antibody
适用: 小鼠, 人, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-IKK-alpha /IKK-beta (AA 151-200), (pSer180) antibody适用: 小鼠, 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-IKK-alpha /IKK-beta (AA 151-250) antibody适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-IKK-alpha /IKK-beta (Lys599) antibody适用: 小鼠, 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-IKK-alpha /IKK-beta (pSer176), (pSer180) antibody适用: 小鼠, 人 WB 宿主: 兔 Polyclonal unconjugated
anti-IKK-alpha /IKK-beta (pSer176), (pSer177) antibody适用: 小鼠, 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-IKK-alpha /IKK-beta (pSer180), (pSer181) antibody适用: 小鼠, 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-IKK-alpha /IKK-beta (Lys6) antibody适用: 小鼠, 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway." in: Life sciences, Vol. 144, pp. 185-93, (2016) (PubMed).
: "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." in: Acta histochemica, Vol. 118, Issue 6, pp. 606-14, (2016) (PubMed).
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: "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway." in: Life sciences, Vol. 144, pp. 185-93, (2016) (PubMed).
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- 抗原
- IKK-alpha /IKK-beta
- 别名
- IKK alpha/beta / (IKK-alpha /IKK-beta 产品)
- 背景
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Synonyms: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha, Transcription factor 16, TCF-16, CHUK
Background: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.
- 基因ID
- 1147
- UniProt
- O15111
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