BrdU 抗体 (Biotin)
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北京 101111
Quick Overview for BrdU 抗体 (Biotin) (ABIN7867111)
抗原
See all BrdU 抗体宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-5-bromodeoxyuridine (BrdU)-Biotin Conjugated Monoclonal Antibody (Clone:Bu20a)
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纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
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免疫原
- Bromodeoxyuridine conjugated to BSA
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亚型
- IgG1
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应用备注
- Flow cytometry: Recommended dilution: 1-5 μg/mL.
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限制
- 仅限研究用
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缓冲液
- 1 mg/mL, Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- BrdU (Bromodeoxyuridine (BrdU))
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别名
- 5-bromodeoxyuridine (BrdU)
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物质类
- Chemical
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背景
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Synonyms: 5-bromodeoxyuridine, Bu20a
Target Background: Bromodeoxyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Specificity: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.
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基因ID
- 35
抗原
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