ST6GAL1 抗体
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北京 101111
Quick Overview for ST6GAL1 抗体 (ABIN7865739)
抗原
See all ST6GAL1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-CDw75 mouse monoclonal, LN-1, purified
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纯化方法
- affinity chromatography
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免疫原
- nuclei from pokeweed mitrogen-stimulated PBL
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亚型
- IgM kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))
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别名
- CDw75
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背景
- LN-1 reacts with CDw75, a neuraminidase sensitive cell surface sialoglycan which is present on the cell membrane and cytoplasm of germininal center B-cells and derived lymphomas. CDw75 has a function in cell adhesion and is the ligand for CD22. LN-1 reacts with RBC precursors in bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with gioblastomas and astrocytoma’s, and also Reed Sternberg cells in Hodgkin’s disease. Positive control: HeLa or Daudi cells. Germinal center B-cell in a lymph node or tonsil. Use LN-1’s ability to stain the surface of erythrocytes as an internal positive control.
抗原
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