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ST6GAL1 抗体

The 小鼠 单克隆 anti-ST6GAL1 antibody is suitable to detect ST6GAL1 in samples from 人. It has been validated for IHC, FACS 和 IF.
产品编号 ABIN6940578
发货至: 中国
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Quick Overview for ST6GAL1 抗体 (ABIN6940578)

抗原

See all ST6GAL1 抗体
ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This ST6GAL1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

LN-1
  • 原理

    CDw75 (B-Cell Marker) Antibody

    特异性

    Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

    纯化方法

    200ug/ml of Ab Purified from Bioreactor Concentrate by PEG precipitation.

    免疫原

    Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

    亚型

    IgM, kappa
  • 应用备注

    Positive Control: HeLa or Daudi cells (IF/FACS). Germinal center B- cells in a lymph node or tonsil (IHC). Use LN1 s ability to stain surface of erythrocytes as an internal positive control.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

    别名

    ST6GAL1

    背景

    6-sialyltransferase 1 (6-ST1), Alpha 2,6 ST1, Beta galactoside alpha 2,6 sialyltransferase 1, CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, SIAT1, ST6 beta galactosamide alpha 2,6 sialyltranferase 1, ST6Gal I, ST6N

    HeLa or Daudi cells (IF/FACS). Germinal center B- cells in a lymph node or tonsil (IHC). Use LN1 s ability to stain surface of erythrocytes as an internal positive control.

    分子量

    46kDa

    基因ID

    6480, 207459

    UniProt

    P15907
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