PLAP 抗体
Our Local Distributor
北京 101111
Quick Overview for PLAP 抗体 (ABIN7865713)
抗原
See all PLAP (ALPP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- anti-Placental alkaline phosphatase (PLAP) mouse monoclonal, H7E8, purified
-
纯化方法
- affinity chromatography
-
免疫原
- PLAP, purified from FS phenotype human placenta
-
亚型
- IgG2a kappa
-
-
anti-Placental Alkaline Phosphatase (ALPP) (AA 162-312) antibody
ALPP 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (AA 403-433), (C-Term) antibodyALPP 适用: 人 WB, IHC, FACS, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal ALP-870 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 WB, IHC 宿主: 兔 Monoclonal ALPP-2899R unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (full length) antibodyALPP 适用: 人 IHC 宿主: 兔 Monoclonal 350R unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (N-Term) antibodyALPP 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyKO Validated ALPP 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB4200 unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal GM022 unconjugated
-
-
应用备注
-
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS with 0.02 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- store at 2 - 8 °C
-
-
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
-
别名
- Placental alkaline phosphatase (PLAP)
-
背景
- PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non tissue specific AP is found in kidney, liver and bone. H7E8 binds equally well to all common allelic variants (S,F, FS and I) of PLAP as to AP from normal human testis, while antibody F5C2 reacts with some samples of normal human testis only. H7E8 can be used as epitope-defined marker in germ cell tumors and is applicable on frozen sections directly as well as on paraffin sections after a heat induced antigen retrieval step. H7E8 can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes. H7E8 can also be used for immunosorbent purification and for radioimmuno-localization. Positive control: human placenta.
抗原
-
