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Laminin gamma 1 抗体

The 大鼠 单克隆 anti-Laminin gamma 1 antibody is suitable to detect Laminin gamma 1 in samples from 人, 小鼠, 大鼠, 猴 和 Pig. It has been validated for WB, IHC, IF, ICC 和 FACS.
产品编号 ABIN7865685
发货至: 中国
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Quick Overview for Laminin gamma 1 抗体 (ABIN7865685)

抗原

See all Laminin gamma 1 (LAMC1) 抗体
Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

适用

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人, 小鼠, 大鼠, 猴, Pig

宿主

  • 68
  • 11
  • 4
大鼠

克隆类型

  • 44
  • 39
单克隆

标记

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This Laminin gamma 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

克隆位点

A5
  • 原理

    anti-Laminin γ1 rat monoclonal, A5, purified

    纯化方法

    affinity chromatography

    免疫原

    laminin preparation from murine EHS cells

    亚型

    IgG2a kappa
  • 应用备注

    ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

    FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

    IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

    WB_dilution: 1:50-1:100 (1-2 μg/mL)

    IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

    别名

    Laminin gamma1

    背景

    Laminins are large hetero-trimeric, non-collagenous glycoproteins found in basement membranes and composed of α, β, and γ chains. A5 reacts specifically with γ chain 1. Alterations of basement membrane integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. Positive control: Normal colon or carcinomas.
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