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KRT14 抗体 (C-Term)

This 小鼠 单克隆 antibody specifically detects KRT14 in IHC, WB, IF, FACS 和 ICC. It exhibits reactivity toward 人, 大鼠, Pig 和 Cat.
产品编号 ABIN7865678
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for KRT14 抗体 (C-Term) (ABIN7865678)

抗原

See all KRT14 抗体
KRT14 (Keratin 14 (KRT14))

适用

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人, 大鼠, Pig, Cat

宿主

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小鼠

克隆类型

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单克隆

标记

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This KRT14 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

克隆位点

EBS-IF-004
  • 抗原表位

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    AA 459-472, C-Term

    原理

    anti-Keratin 14 mouse monoclonal, EBS-IF-004, purified

    纯化方法

    affinity chromatography

    免疫原

    AA 459-472 at the carboxyterminal end of human cytokeratin 14, conjugated to KLH

    亚型

    IgM kappa
  • 应用备注

    ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

    FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

    IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

    WB_dilution: 1:50-1:100 (1-2 μg/mL)

    IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    KRT14 (Keratin 14 (KRT14))

    别名

    Keratin 14

    背景

    EBS-IF-004 reacts specifically with keratin 14 (50 kDa) in immunoblotting. In tissue sections EBS-IF-004 is positive with basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas. Positive control: Skin.
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