KRT14 抗体 (C-Term)
Our Local Distributor
北京 101111
Quick Overview for KRT14 抗体 (C-Term) (ABIN7865678)
抗原
See all KRT14 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 459-472, C-Term
-
原理
- anti-Keratin 14 mouse monoclonal, EBS-IF-004, purified
-
纯化方法
- affinity chromatography
-
免疫原
- AA 459-472 at the carboxyterminal end of human cytokeratin 14, conjugated to KLH
-
亚型
- IgM kappa
-
-
-
-
应用备注
-
ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS with 0.02 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- store at 2 - 8 °C
-
-
- KRT14 (Keratin 14 (KRT14))
-
别名
- Keratin 14
-
背景
- EBS-IF-004 reacts specifically with keratin 14 (50 kDa) in immunoblotting. In tissue sections EBS-IF-004 is positive with basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas. Positive control: Skin.
抗原
-