kappa Light Chain 抗体
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北京 101111
Quick Overview for kappa Light Chain 抗体 (ABIN7865676)
抗原
See all kappa Light Chain 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Kappa-Light-Chain mouse monoclonal, EBS-huKappa, purified
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交叉反应 (详细)
- negative with rat
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纯化方法
- affinity chromatography
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免疫原
- B lymphoma cells
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亚型
- IgG1 kappa
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应用备注
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ICC/IF_dilution: 1:100 - 1:200 (0.5 - 1.0 μg/mL)
FACS_dilution: 0.5 - 1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50 - 1:100 (1 - 2 μg/mL, microwave treatment recommended)
IHC-frozen_dilution: 1:50 - 1:100 (1 - 2 μg/mL)
elisa_dilution: 1:1 - 1:100,000 (0.001 - 100 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0,02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- kappa Light Chain
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别名
- Kappa-Light-Chain
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背景
- EBS-huKappa reacts with kappa light chain In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicate tha the infiltrate is malignant. Positive control: 293T, Raji or hPBL cells, tonsil or spleen.
抗原
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