(5 references)-
- 抗原 See all kappa Light Chain 抗体
- kappa Light Chain
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This kappa Light Chain antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- Human IgG-kappa myeloma protein
- 克隆位点
- TB28-2
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product kappa Light Chain Primary Antibody
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anti-kappa Light Chain antibody (FITC)
适用: 人 FACS 宿主: 小鼠 Monoclonal A8B5 FITC
anti-kappa Light Chain antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 PE
anti-kappa Light Chain antibody适用: 人 FACS, IHC (p), ELISA, IHC (fro) 宿主: 小鼠 Monoclonal A8B5 unconjugated
anti-kappa Light Chain antibody适用: 人 FACS, WB 宿主: 小鼠 Monoclonal TB28-2 unconjugated
anti-kappa Light Chain antibody适用: 人 FACS, WB, IHC (p), ELISA, ICC, IP 宿主: 小鼠 Monoclonal MEM-09 unconjugated
anti-kappa Light Chain antibody (Biotin)适用: 人 FACS 宿主: 小鼠 Monoclonal TB28-2 Biotin
anti-kappa Light Chain antibody (mFluor™450)GMP Grade 适用: 人 宿主: 小鼠 Monoclonal TB28-2 mFluor™450
anti-kappa Light Chain antibody (PE-Cy7)GMP Grade 适用: 人 FACS 宿主: 小鼠 Monoclonal TB 28-2 PE-Cy7
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- 应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
- 说明
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The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- 限制
- 仅限研究用
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- 溶解方式
- No reconstitution is necessary.
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).
: "Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for ..." in: Haematologica, Vol. 93, Issue 4, pp. 551-9, (2008) (PubMed).
: "Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis." in: American journal of clinical pathology, Vol. 116, Issue 2, pp. 204-10, (2001) (PubMed).
: "Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8522-6, (1992) (PubMed).
: "Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4150-8, (1987) (PubMed).
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: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).
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- 抗原
- kappa Light Chain
- 别名
- Kappa light chains (kappa Light Chain 产品)
- 别名
- LOC100037688 antibody, B128L antibody, B55L antibody, IgK antibody, TCRAV31S1 antibody, TCRAV40S1 antibody, kappa light chain antibody, immunoglobulin kappa chain variable 8 (V8) antibody, T cell receptor alpha variable 40 antibody, LOC100037688 antibody, Igk-V8 antibody, TRAV40 antibody
- 背景
- Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin kappa, Igk
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