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Integrin beta 2 抗体

The 小鼠 单克隆 anti-Integrin beta 2 antibody (Clone 68-5A5) (ABIN7865662) specifically detects Integrin beta 2 in FACS, WB, IHC, IF 和 ICC. The antibody is reactive with 人 samples.
产品编号 ABIN7865662
发货至: 中国
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Quick Overview for Integrin beta 2 抗体 (ABIN7865662)

抗原

See all Integrin beta 2 (ITGB2) 抗体
Integrin beta 2 (ITGB2)

适用

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小鼠

克隆类型

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单克隆

标记

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This Integrin beta 2 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

68-5A5
  • 原理

    anti-CD18 mouse monoclonal, 68-5A5, purified

    纯化方法

    affinity chromatography

    免疫原

    stimulated human leucocytes

    亚型

    IgG2a kappa
  • 应用备注

    ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

    FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

    WB_dilution: 1:50-1:100 (1-2 μg/mL)

    IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    Integrin beta 2 (ITGB2)

    别名

    CD18

    背景

    It recognizes a transmembrane glycoprotein of 95 kDa, identified as CD18 or intergrin-2 (Workshop III). It complexes non-covalently with either L, M, or X integrin (CD11a, b, or c) to form the heterodimers. LFA-1, MAC-1, and p150,95, respectively. LFA-1 is the receptor for three members of the Ig supergene family of proteins, ICAM-1 (CD54, ICCAM-2 (CD102), and Mac-1 and p150,95 bind to ICAM-1, fibrinogen, and IC3b. ICAM-3 (CD50). CD18/CD11 heterodimeric molecules are involved with cell/cell and cell/extracellular adhesion in immune and inflammatory responses. This Mab blocks these cellular interactions. 68-5A5 was clustered at the IIIrd International Workshop on human leucocyte differentiation antigens. Positive control: Human PBL and tonsil.

    途径

    NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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