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CD99 抗体

The 小鼠 单克隆 anti-CD99 antibody (Clone EBS-CD-044) (ABIN7865611) specifically detects CD99 in IHC, WB, FACS, IF 和 ICC. The antibody is reactive with 人 samples.
产品编号 ABIN7865611
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD99 抗体 (ABIN7865611)

抗原

See all CD99 抗体
CD99

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD99 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

EBS-CD-044
  • 原理

    anti-CD99 mouse monoclonal, EBS-CD-044, purified

    纯化方法

    affinity chromatography

    免疫原

    purified E-rosette forming cells from human PBL

    亚型

    IgM kappa
  • 应用备注

    ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

    FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,02 % saponin or digitonin for 15 min at 4 °C

    IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

    WB_dilution: 1:50-1:100 (1-2 μg/mL)

    IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS with 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    store at 2 - 8 °C
  • 抗原

    CD99

    别名

    CD99

    背景

    EBS-CD-044 reacts with CD99 or MIC2. Human thymocytes, PBLs and some T-ALL isolates and cell lines are positive. It is also present on pancreas and on Ewing sarcoma, which forms the practical application of the antibody. It is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Positive control: Pancreas, thymus, Ewing sarcoma.
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