CD59 抗体
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北京 101111
Quick Overview for CD59 抗体 (ABIN7865599)
抗原
See all CD59 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-CD59 mouse monoclonal, Bra10G, purified
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纯化方法
- affinity chromatography
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免疫原
- K-562 leukemia cells
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亚型
- IgG2b kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- CD59
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别名
- CD59
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背景
- CD59, or protectin, is a 18-22 kDa cell surface molecule on a GPI anchor. It regulates complement-mediated cell lysis and is supposed to protect normal and tumor cells from cytotoxic attack by homologous complement through binding to C8 and C9. CD59 is expressed on leukocytes, vascular epithelium, a variety of epithelial cells and placenta. B-cells express low levels. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease Paroxysmal nocturnal hemoglobinuria (PNH). Bra10G was typed at the VIth International Workshop on human leucocyte differentiation antigens. Positive control: Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, human lymphocytes, human lymph node and tonsil.
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途径
- Complement System
抗原
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