CEACAM5 抗体
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北京 101111
Quick Overview for CEACAM5 抗体 (ABIN7865572)
抗原
See all CEACAM5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-CD66e (CEACAM5) mouse monoclonal, CB-30, purified
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纯化方法
- affinity chromatography
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免疫原
- human colon cancer extract
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亚型
- IgG1 kappa
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应用备注
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
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别名
- CD66e (CEACAM5)
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背景
- CB-30 reacts with CD66e or CEA with MW of 80-200 kDa. CEA is present in fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Ant-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbladder, urachus, salivary gland, ovary, and endocervix. Positive control: MCF7 cells, 293T cells, colon carcinoma.
抗原
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