Recombinant NeuN 抗体
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Quick Overview for Recombinant NeuN 抗体 (ABIN7862669)
抗原
See all NeuN (RBFOX3) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Neuronal-Nuclei (NeuN) (Neuronal Marker) Antibody
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特异性
- NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function, however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant full-length human NeuN protein
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亚型
- IgG, kappa
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anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody
RBFOX3 适用: 人, 小鼠 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1H3B2 unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-220) antibodyRBFOX3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibodyRBFOX3 适用: 人, 小鼠 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5A4B9 unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 51-150) antibodyRBFOX3 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 28-304) antibodyRBFOX3 适用: 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyRBFOX3 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (C-Term) antibodyRBFOX3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
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别名
- Neuronal-Nuclei
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背景
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FLJ56884, FLJ58356, fox1 homolog C, Fox3, FOX3NeuN, hexaribonucleotide binding protein 3 (HRNBP3), neuronal nuclei, Rbfox3, RNA binding protein, fox 1 homolog (C. elegans) 3
Human brain. HeLa cells.
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分子量
- 48kDa
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基因ID
- 146713, 135229
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UniProt
- A6NFN3
抗原
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