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NFIA 抗体

This 小鼠 单克隆 antibody specifically detects NFIA in WB, IHC, IF 和 FACS. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN7863189
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for NFIA 抗体 (ABIN7863189)

抗原

See all NFIA 抗体
NFIA (Nuclear Factor I/A (NFIA))

适用

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人, 小鼠, 大鼠

宿主

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小鼠

克隆类型

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  • 9
单克隆

标记

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This NFIA antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

PCRP-NFIA-2C6
  • 原理

    NFIA / NF1A (Nuclear Factor 1A) (Transcription Factor) Antibody

    特异性

    NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, °™-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant fragment of human NFIA protein corresponding to protein domain

    亚型

    IgG2c, kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    NFIA (Nuclear Factor I/A (NFIA))

    别名

    NFIA

    背景

    CCAAT-box-binding transcription factor, CTF, NF I/A, NF-I/A, NF1-A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1 A-type, TGGCA binding protein

    Colon, HeLa cells. Human fetal gut, liver or heart.

    分子量

    55kDa

    基因ID

    4774, 322431

    UniProt

    Q15784
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