Nidogen 抗体
The 大鼠 单克隆 anti-Nidogen antibody (Clone ELM1) (ABIN7861825) specifically detects Nidogen in ELISA.
The antibody is reactive with 小鼠 和 豚鼠 samples.
产品编号 ABIN7861825
发货至:
中国
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Quick Overview for Nidogen 抗体 (ABIN7861825)
抗原
Nidogen (NID)
适用
小鼠, 豚鼠
宿主
大鼠
克隆类型
单克隆
标记
This Nidogen antibody is un-conjugated
应用范围
ELISA
克隆位点
ELM1
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原理
- Nidogen / Entactin Antibody
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特异性
- It reacts with the intact (150 kDa) nidogen as well as its proteolytic fragments of 135 kDa, 110 kDa, 100 kDa, and 80 kDa. This MAb does not cross-react with other basement membrane components or fibronectin. Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Murine EHS laminin preparation
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亚型
- IgG2a, kappa
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anti-Nidogen (NID) (AA 971-1219) antibody
NID 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Nidogen (NID)
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别名
- Nidogen
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背景
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Nid1, Ent, Entactin 1, NID-1, Nidogen-1,
Mouse kidney or eye tissue.
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分子量
- 100|105|110|135|and 80kDa
抗原
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