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EIF4E 抗体

This 小鼠 单克隆 antibody specifically detects EIF4E in WB, IF 和 FACS. It exhibits reactivity toward 人.
产品编号 ABIN7862735
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EIF4E 抗体 (ABIN7862735)

抗原

See all EIF4E 抗体
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

适用

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宿主

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小鼠

克隆类型

  • 100
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单克隆

标记

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This EIF4E antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

PCRP-EIF4E-1D3
  • 原理

    EIF4E Antibody

    特异性

    The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA cap during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant full-length human EIF4E protein

    亚型

    IgG2a
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    别名

    EIF4E

    背景

    AUTS19, CBP, eIF-4F 25 kDa subunit, eIF4E, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E like 1, Messanger RNA (mRNA) Cap Binding Protein eIF 4E

    HeLa, Raji or U87 cells.

    分子量

    25.1kDa

    基因ID

    1977, 249718

    UniProt

    P06730

    途径

    BCR Signaling
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