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Recombinant Caldesmon HMW 抗体

This 小鼠 单克隆 antibody specifically detects Caldesmon HMW in WB. It exhibits reactivity toward 人.
产品编号 ABIN7860956
发货至: 中国
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Quick Overview for Recombinant Caldesmon HMW 抗体 (ABIN7860956)

抗原

Caldesmon HMW

抗体类型

Recombinant Antibody

适用

  • 32
  • 11

宿主

  • 20
  • 12
小鼠

克隆类型

  • 30
  • 2
单克隆

标记

  • 11
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
非结合性

应用范围

  • 29
  • 29
  • 18
  • 10
  • 4
  • 3
Western Blotting (WB)

克隆位点

RCALD1-820
  • 原理

    Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody

    特异性

    Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant full-length human CALD1 protein

    亚型

    IgG1, kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    Caldesmon HMW

    别名

    Caldesmon, HMW

    背景

    CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

    Blood vessels in all tissues, ovary, smooth muscle or leiomyosarcoma, Uterus

    分子量

    120-150kDa

    基因ID

    800, 490203

    UniProt

    Q05682
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