Recombinant SMAD4 抗体 (AA 400-552)
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Quick Overview for Recombinant SMAD4 抗体 (AA 400-552) (ABIN7864839)
抗原
See all SMAD4 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 400-552
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原理
- Recombinant SMAD4 / DPC4 (Pancreatic Adenocarcinoma Marker / Tumor Suppressor) Antibody
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特异性
- Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4. Upon ligand binding, the receptors of the TGF-≤ family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80 % of cases of pancreatic adenocarcinoma, but rarely in endometrial (0 %), colorectal (0 %), ovarian (3 %), lung (0 %), breast (2 %) adenocarcinomas, and malignant melanoma (4 %). SMAD4is an important marker for confirming a diagnosis of pancreatic adenocarcinoma.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant fragment (around aa400-552) of human SMAD4 protein (exact sequence is proprietary)
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亚型
- IgG, kappa
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anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody
SMAD4 适用: 人 WB, IP, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 1-552) antibodySMAD4 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 3E2 unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodySMAD4 适用: 人, 小鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4G1C6 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 255-284) antibodySMAD4 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB14714 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 400-428) antibodySMAD4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 27-195) antibodySMAD4 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) antibodyKD Validated SMAD4 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 23GB975 unconjugated Recombinant Antibody
anti-SMAD Family Member 4 (SMAD4) (AA 400-428), (C-Term) antibodySMAD4 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB20720 unconjugated
anti-SMAD Family Member 4 (SMAD4) (AA 40-120) antibodySMAD4 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD Family Member 4 (SMAD4) (pThr277) antibodySMAD4 适用: 人 ELISA, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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- SMAD4 (SMAD Family Member 4 (SMAD4))
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别名
- SMAD4
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背景
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Deleted in Pancreatic Carcinoma 4 (DPC4), JIP, MAD homolog 4 (MADH4), Mothers against decapentaplegic homolog 4 (MADH4), Mothers against DPP homolog 4, MYHRS, SMAD4
HeLa, Human pancreatic ductal adenocarcinoma.
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分子量
- 61kDa
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基因ID
- 4089, 75862
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途径
- Cell Division Cycle, Chromatin Binding, Autophagy
抗原
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