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Recombinant CD34 抗体 (AA 100-300)

The 小鼠 单克隆 anti-CD34 antibody is suitable to detect CD34 in samples from 人. It has been validated for WB 和 IHC.
产品编号 ABIN7863990
发货至: 中国
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Our Local Distributor

中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant CD34 抗体 (AA 100-300) (ABIN7863990)

抗原

See all CD34 抗体
CD34

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD34 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

RHPCA1-8573
  • 抗原表位

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    AA 100-300

    原理

    Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody

    特异性

    This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intoÇ 79oΩthree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.Ç 79oΩCD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant fragment (around aa100-300) of human HPCA1 protein (exact sequence is proprietary)

    亚型

    IgG1, lambda
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    200 μg/mL

    缓冲液

    Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    CD34

    别名

    CD34

    背景

    Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

    Human tonsil or angiosarcoma. Heart.

    分子量

    90-110kDa

    基因ID

    947, 374990

    UniProt

    P28906
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