Recombinant CD34 抗体 (AA 100-300)
Our Local Distributor
北京 101111
Quick Overview for Recombinant CD34 抗体 (AA 100-300) (ABIN7863990)
抗原
See all CD34 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 100-300
-
原理
- Recombinant CD34 (Hematopoietic Stem Cell & Endothelial Marker) Antibody
-
特异性
- This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intoÇ 79oΩthree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.Ç 79oΩCD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.
-
纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
-
免疫原
- Recombinant fragment (around aa100-300) of human HPCA1 protein (exact sequence is proprietary)
-
亚型
- IgG1, lambda
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- CD34
-
别名
- CD34
-
背景
-
Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin
Human tonsil or angiosarcoma. Heart.
-
分子量
- 90-110kDa
-
基因ID
- 947, 374990
-
UniProt
- P28906
抗原
-