IRS1 抗体 (AA 1-200)
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北京 101111
Quick Overview for IRS1 抗体 (AA 1-200) (ABIN7858544)
抗原
See all IRS1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-200
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原理
- IRS1 (Insulin receptor substrate 1) Antibody
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特异性
- The Insulin receptor substrate-1 (IRS-1), a protein major substrate of the Insulin receptor, is phosphorylated in response to stimulation of cells by Insulin, Insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An Insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the Insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase IIcatalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase IIcatalyzed phosphorylation of IRS-1 may be a component of the intracellular Insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the Insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of Insulin sensitivity by interrupting the association between the Insulin receptor and IRS-1.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant fragment (around aa1-200) of human IRS1 protein (exact sequence is proprietary)
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亚型
- IgG2, kappa
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anti-Insulin Receptor Substrate 1 (IRS1) (pSer312) antibody
IRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibodyIRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (pSer639) antibodyIRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) antibodyIRS1 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (AA 1041-1242) antibodyIRS1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (pSer307) antibodyIRS1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (AA 106-122), (N-Term) antibodyIRS1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (Ser312) antibodyIRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (pSer636) antibodyIRS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Insulin Receptor Substrate 1 (IRS1) (AA 1041-1242) antibodyIRS1 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 小鼠 Monoclonal 10I3 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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- IRS1 (Insulin Receptor Substrate 1 (IRS1))
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别名
- IRS1
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背景
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HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1, IRS1_HUMAN, OTTHUMP00000164234
adrenal gland or lung.|Human breast carcinoma
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分子量
- 170-185kDa
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基因ID
- 3667, 445868
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
抗原
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