Recombinant Nucleolin 抗体
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Quick Overview for Recombinant Nucleolin 抗体 (ABIN7863187)
抗原
See all Nucleolin (NCL) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Expression System
- HEK-293 Cells
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原理
- Recombinant Nucleolin (Marker of Human Cells) Antibody
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特异性
- Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
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纯化方法
- 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.
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免疫原
- Recombinant full-length human NCL protein
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亚型
- IgG, kappa
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anti-Nucleolin (NCL) (AA 353-568) antibody
NCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Nucleolin (NCL) (AA 388-569) antibodyNCL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IHC, IF, FACS, ISt 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 364-5-NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyCarrier-free NCL 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) antibodyNCL 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal NCL-902 unconjugated
anti-Nucleolin (NCL) (AA 425-455) antibodyNCL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleolin (NCL) (AA 428-455) antibodyNCL 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB17464 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Nucleolin (NCL)
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别名
- Nucleolin
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背景
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NCL, Nucl, Nucleolin, Protein C23
All human cells. Human testis, ovary or Hodgkin s lymphoma.
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分子量
- 76kDa
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基因ID
- 4691, 79110
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UniProt
- P19338
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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