CD35 抗体 (PE)
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北京 101111
Quick Overview for CD35 抗体 (PE) (ABIN7836199)
抗原
See all CD35 (CR1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody PE labled to CD35
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特异性
- Recognizes human CD35
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Native purified human CD35.
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anti-Complement Component Receptor 1 (CD35) (CR1) (AA 1780-2039) antibody
CR1 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 420-550) antibodyCR1 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, 小鼠, 大鼠 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, Non-Human Primate FACS, IHC (p), IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal E11 unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibody (FITC)CR1 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal E11 FITC
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 420-550) antibodyCR1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 901-1095) antibodyCR1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 9A9 unconjugated Recombinant Antibody
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, Baboon, Cynomolgus, 猕猴 FACS, WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal E11 unconjugated
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应用备注
- Flow: 10 μL / assay
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Shipped and store at 4°C for one year. Do not freeze.
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- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
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别名
- CR1
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背景
- C3BR, Complement receptor type 1, C3b/C4b receptor, CD antigen CD35
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基因ID
- 1378
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UniProt
- P17927
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途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
抗原
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