RBCL 抗体 (HRP)
The HRP-conjugated 兔 多克隆 anti-RBCL antibody (ABIN7833500) specifically detects RBCL in ELISA 和 WB.
The antibody is reactive with Arabidopsis thaliana, Soybean, Alfalfa, Synechococcus sp., Spinach, Pea, Kochia prostrata, Physcomytrium Patens, Phaeodactylum tricornutum, Petunia hybrida, Panicum virgatum, Nicotiana tabacum, Nicotiana benthamiana, Micromonas pusilla, Methylacidiphilum fumariolicum, Liquidambar formosana, Lathyrus sativus, Porosira glacialisPorphyra (Red Algae), Karenia brevis, Kandelia candel, Hypnum cupressiforme, Hevea, Heterosigma akashiwo, Guzmania hybrid, Gonyaulax polyedra, Galdieria sulphuraria, Vitis vinifera, Tradescantia spathacea, Tomato, Verbascum lychnitis, Tisochrysis lutea, Thermosynechococcus elongatus, Thalassiosira punctigera, Thalassiosira pseudonana, Symbiodinium, Stanleya pinnata, Polytrichum formosum, Schima superba, Saccharum, Robinia pseudoacacia, Ricinus communis, Rhytidiadelphus squarrosus, Rape, Quercus ilex, Prochlorococcus, Triticum aestivum, Chlamydomonas raudensis, Apple, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata, Barbados Nut (Jatropha curcas), Barley, Beet, Begonia, Bienertia sinuspersici, Cannabis sativa L., Celery, Chaetoceros neogracilis, Chenopodium quinoa, Chickpea, Cylindrospermopsis raciborskii, Fucus vesiculosus, Fraxinus mandshurica, Fortunella margarita, Ficus, Euglena gracilis, Emiliania huxleyi, Diatom (Skeletonema), Cynara cardunculus, Chlamydomonas reinhardtii, Cyanophora paradoxa, Coscinodiscus concinnus, Colobanthus quitensis, Chlorococcum sp. 'dorsiventrale', Chlorella vulgaris, Chlorella sorokiniana 和 Agrostis stolonifera samples.
产品编号 ABIN7833500
发货至:
中国
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Quick Overview for RBCL 抗体 (HRP) (ABIN7833500)
抗原
RBCL
适用
Arabidopsis thaliana, Soybean, Alfalfa, Synechococcus sp., Spinach, Pea, Kochia prostrata, Physcomytrium Patens, Phaeodactylum tricornutum, Petunia hybrida, Panicum virgatum, Nicotiana tabacum, Nicotiana benthamiana, Micromonas pusilla, Methylacidiphilum fumariolicum, Liquidambar formosana, Lathyrus sativus, Porosira glacialisPorphyra (Red Algae), Karenia brevis, Kandelia candel, Hypnum cupressiforme, Hevea, Heterosigma akashiwo, Guzmania hybrid, Gonyaulax polyedra, Galdieria sulphuraria, Vitis vinifera, Tradescantia spathacea, Tomato, Verbascum lychnitis, Tisochrysis lutea, Thermosynechococcus elongatus, Thalassiosira punctigera, Thalassiosira pseudonana, Symbiodinium, Stanleya pinnata, Polytrichum formosum, Schima superba, Saccharum, Robinia pseudoacacia, Ricinus communis, Rhytidiadelphus squarrosus, Rape, Quercus ilex, Prochlorococcus, Triticum aestivum, Chlamydomonas raudensis, Apple, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata, Barbados Nut (Jatropha curcas), Barley, Beet, Begonia, Bienertia sinuspersici, Cannabis sativa L., Celery, Chaetoceros neogracilis, Chenopodium quinoa, Chickpea, Cylindrospermopsis raciborskii, Fucus vesiculosus, Fraxinus mandshurica, Fortunella margarita , Ficus, Euglena gracilis, Emiliania huxleyi, Diatom (Skeletonema), Cynara cardunculus, Chlamydomonas reinhardtii, Cyanophora paradoxa, Coscinodiscus concinnus, Colobanthus quitensis, Chlorococcum sp. 'dorsiventrale', Chlorella vulgaris, Chlorella sorokiniana, Agrostis stolonifera
宿主
兔
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Western Blotting (WB)
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原理
- Anti-RbcL | Rubisco large subunit, form I, HRP-conjugated
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交叉反应 （详细）
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Agostis stolonifera cv. Penncross, Arabidopsis thaliana, Apium graveolens, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata (benthic foraminifer), Beta vulgaris, Begonia sp., Bienertia sinuspersici, Brassica napus, Kandelia candel, Cannabis sativa L., Chaetoceros furcellatus, Chlorococcum dorsiventrale, Colobanthus quitensis, Cicer arietinum, Chenopodium quinoa, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Chlorella sorokiniana, Chlorella vulgaris, Coscinodiscus concinnus, Cyanophora paradoxa, Cylindrospermopsis raciborskii CS-505, Cynara cardunculus, Emiliana huxleyi, Euglena gracilis, Ficus carica, Fortunella margarita Swingle, Fraxinus mandshurica, Fucus vesiculosus, Gladieria sulphuraria, Glycine max, Gonyaulax polyedra, Guzmania hybrid, Heterosigma akashiwo, Hevea, Hordeum vulgare, Hypnum cupressiforme, Jatropha curcas, Karenia brevis (C.C.Davis) s) G.Hansen & U.Moestrup (Wilson isolate), Kochia prostrata, Lathyrus sativus, Liquidambar formosana, Malus domestica, Medicago truncatula, Micromonas pusila, Nicotiana benthamiana, Nicotiana tabacum, Panicum virgatum, Petunia hybrida cv. Mitchell, Phaeodactylum tricornutum, Physcomitrium patens, Pisum sativum, olytrichum formosum, Porosira glacialis, Porphyra sp., Ricinus communis, Robinia pseudoacacia, Rhytidiadelphus squarrosus, Saccharum sp., Schima superba, Skeletonema costatum (diatom), Skeletonema marinoi (diatom), Solanum lycopersicum, Spinacia oleracea, lichens, Stanleya pinnata, Symbiodinium sp., Synechococcus PCC 7942, Synechococcus elongatus UTEX 2973, Rhoeo discolor, Thalassiosira pseudonana, Thermosynechococcus elongatus, Triticum aestivum, Prochlorococcus sp. (surface and deep water ecotype), Triticum aestivum, dinoflagellate endosymbionts (genus Symbiodinium), extreme acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum strain SolV, Thalassiosira punctigera, Tisochrysis lutea, Verbascum lychnitis, Vitis vinifera, Quercus ilex
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Alpha proteobacteria,Algae (brown,red) including Galdieria sulphuraria,Dicots,Benincasa hispida,Kalanchoe fedtschenkoi, Beta-proteobacteria,Conifers,Cryptomonads,Cyanobacteria (prochlorophytes),Gamma-proeobacteria,Liverworts,Manihot esculenta,Marchantia polymorpha,Monocots,Mosses,Suaeda glauca,Welwitschia, Nannochloropsis sp.,Picochlorum sp.,Porphyridium purpureum,Zea mays,Zosteria marinaFor detection in Rhodospirillaceae use product AS15 2955
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated synthetic peptide conserved across all known plant
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anti-RBCL (AA 3-475) antibody
适用: Soybean ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-RBCL (AA 3-479) antibody适用: Arabidopsis thaliana ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 5000 (ELISA), 1 : 10 000-25 000 (WB)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
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注意事项
- A preservative may be added for long time storage
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储存条件
- 4 °C
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储存方法
- Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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有效期
- 12-24 months
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- RBCL
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背景
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Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.This antibody is directly conjugated to HRP.
Additional information: Anti-RbcL can be used as a cellular [compartment marker] of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.
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分子量
- 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
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基因ID
- 844754
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UniProt
- O03042, P05698, P0C510, P00877
抗原
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