RBCL (Isoform 1) 抗体
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北京 101111
Quick Overview for RBCL (Isoform 1) 抗体 (ABIN93524)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Isoform 1
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原理
- Anti-RbcL | Rubisco large subunit, form I (chicken)
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交叉反应 （详细）
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Arabidopsis thaliana, Fragilariopsis cylindrus, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Microcystis aeruginosa PCC7806, Pinus strobus, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Algae,Dicots,Conifers,Liverworts,Mosses,Prochlorophytes,Welwitschia
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纯化方法
- Purified, total IgY
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免疫原
- KLH-conjugated synthetic peptide derived from all known plant
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anti-RBCL (AA 3-475) antibody
适用: Soybean ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-RBCL (AA 3-479) antibody适用: Arabidopsis thaliana ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 μg of total cellular protein, (WB)
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说明
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This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 16 µg/µL
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缓冲液
- Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- 4 °C
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储存方法
- Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, (2008) (PubMed).
: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).
: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." in: PLoS ONE, Vol. 3, Issue 10, pp. e3570, (2008) (PubMed).
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- RBCL
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背景
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Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.
Additional information: Peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria
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分子量
- 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)
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基因ID
- 844754
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UniProt
- O03042, Q3ALL1
抗原
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