mNeonGreen 抗体
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北京 101111
Quick Overview for mNeonGreen 抗体 (ABIN7833426)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-mNeonGreen | Fluorescent Protein
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交叉反应 （详细）
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mNG tag in plant (Arabidopsis thaliana) and algal vectors (Chlamydomonas reinhardtii)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known. -
纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated peptide derived from synthetic peptide
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应用备注
- 1 μg/1 mL (IF), 1 : 1000 - 1: 5000 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL, of sterile water.
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缓冲液
- Immunogen affinity purified serum, in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- mNeonGreen
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背景
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Background: mNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP. It has an excitation maximum at 506 nm and an emission maximum at 517 nm and therefore is compatible with the most GFP filter sets. mNeonGreen is 3x brighter compare to GFP, more stable and does not bleach so fast as GFP, which makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has MW of 26.6 kDa.
Additional information: The peptide used to elicit this antibody is conserved in the following expression vectors: dCas9-P2A-DHFR(TSc3)-T2A-mNeonGreen [Cloning vector pBM020]Cas9-P2A-DHFR(TSc3)-T2A-mNeonGreen [Cloning vector pBM006]mNeonGreen4-tDeg [Cloning vector pminiCMV-mNeonGreen4-tDeg]ER-localized mNEONGREEN [Binary vector pKT-NM-erNEON]mNeonGreen-3xFLAG [Cloning vector pLM160-mNeonGreen]mNeonGreen-ty1 [Cloning vector pSAG1-mNeonGreen_TUB1-dTomato]mNeonGreen, partial [Binary vector pRATIO2131]The peptide used to elicit this antibody is not conserved in GFP protein sequence. Antibody is also recognzing mCheery sequence.
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分子量
- Depends upon used fusion partner
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UniProt
- A0A1S4NYF2
抗原
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